Transformación plásmido CNTN1 Es segueix el protocol de Stratagene: XL10-Gold Ultracompetent cells. Cat nº: 200314 *durant aquesta estona, escalfar l’agitador orbital (37ºC) i el bany…
SAMPLES: EM-158 EM-163 EM-390 EM-223 EM-316 EM-247 EM-307 EM-397 EM-352 (LCR) EM-406 PROCEDURE: Kit Euroimmun (FA-1124-1005-50) Patient’s sera diluted 1/10 in PBS-Tween 0,2% (LCR 1/5)…
SAMPLES: EM-158 EM-163 EM-390 EM-223 EM-316 EM-247 EM-307 EM-397 EM-352 (LCR) EM-406 FLOT1 ICC: HEKs 293 Cells Transfection: 10/10/2020 (normal transfection with lipofectamine and 8…
SAMPLES: 1-8) MMN patients: 09-2582, 09-299, 09-1218, 09-1461, 09-292, 09-190, 09-254, 09-1804 (dil. 1/10) 9) MS 163 (dil. 1/10) 10) MS 402 (dil.1/10) 11) MS…
SAMPLES: First group: 1-23) Miastenia Gravis patients: 190-11, 191-03, 192-04, 192-05, 193-17, 194-07, 194-09, 195-04, 195-15, 197-06, 198-09, 198-15, 199-08, 199-20, 201-07, 202-08, 203-03, 203-05,…
SAMPLES: 1-23) ALS patients: 207-08, 208-17, 210-16, 211-10, 211-11, 214-07, 214-08, 214-13, 214-14, 215-03, 215-09, 215-14, 215-15, 215-16, 218-22, 218-28, 210-02, 219-10, 221-03, 221-08, 222-03,…
Wells: 30 ug protein/well. Marker Rat brain Rat cerebellum non-transfected HEK cells FLOT1/2-transfected HEK cells Marker Rat brain Rat cerebellum non-transfected HEK cells FLOT1/2-transfected HEK…
50 ug protein/well. We use 2 gels. First Gel –> wells: Marker non-transfected HEK cells FLOT1/2-transfected HEK cells Rat brain Rat cerebellum Swine optic nerve…
Wells: 50 ug protein/well Marker non-transfected HEK cells FLOT1/2-transfected HEK cells Rat brain Rat cerebellum Swine optic nerve – Marker non-transfected HEK cells FLOT1/2-transfected HEK…
Lysates: Non-transfected HEK cells FLOT1/2 transfected HEK cells Rat brain Rat cerebellum Swine optic nerve All of them were lysed in RIPA buffer + protease…