2 pozos con HEKs transfectadas Objetivo: hacer fotos de ICC en HEKs no transfectadas para la revisión del paper. Muestras: Protocolo: Resultado: negativo, hago las…
SAMPLES:All the samples are from Lleida patient’s sera22nd: 112, 114, 115, 117, 118, 120, 121, 126, 127, 129, control +, control –23rd: 134, 140, 142,…
SAMPLES: All the samples are from Lleida patient’s sera13th: 17,19, 21, control +, control – 14th: 22, 23, 24, 25, 26, 29, 30, 32, control…
Objective: To test IgG towards ANO2 in MS patients and to check if the sample 153 is positive. Samples: 153 (serum), 328 (LCR), negative control (serum) ICC ANO2 – CONDITION 1 (with primary antibody):…
Objective: To test IgG towards ANO2 in MS patients and to check crossreactivity. Samples: 217; Negative Control ICC ANO2 – CONDITION 1: HEK 293 Cell…
Objective: To test IgG towards ANO2 in LCR from MS patients and to check crossreactivity from the experiment done in 20190211. Samples: ICC ANO2 –…
Objective: To test IgG towards ANO2 in LCR in a patient positive for ANO2 in serum (217) Samples: 328 (LCR) at 1/10 and 1/40 dilution;…
Objective: To check crossreactivity in an MS sample (279) that seemed to be slightly positive for ANO2. Samples: 279, CTRL – 2, CTRL + (217).…
2018.12.19: Prepare: 1 60mm-plate with 26 coverslips of 9mm. 2 100mm-plates (without coverslips) Treatment with Poly-D-Lysine (diluted 1/40 with boratte buffer) Incubate for 1h (37ºC,…
Samples: 300, 301, 302, 305, 306, 307, 309, 311, 312, 315, 318, 322, 323, 324, 325, 326, 327, 317= positive control, negative control ANO2 ICC:…