a) CNTN1/Caspr1 co-transfected HEK (Caspr1 mAb) Blocking solution: goat 5% Patients’ sera diluted 1:100 Ac anti-Caspr1 diluted 1/250 GAH 594 IgG and GAR 488 IgG…
a) CNTN1/Caspr1 co-transfected HEK Blocking solution: goat 5% Patients’ sera diluted 1:100 Ac anti-Caspr1 diluted 1/250 GAH 594 IgG and GAR 488 IgG diluted 1/1000…
OBJECTIVE: to test CNTN1 and Caspr1 expression in Lec1 cells A) Caspr1 transfected Lec1 cells: TO TEST CNTN1 expression: Blocking solution: rabbit serum 1:40 in…
CNTN1 transfected Lec1 cells Trasfection 20190805 Same protocol ICC HEK cells (but without commercial Ab= Samples: CNTN1/Caspr1 positive patients: 17595, 16047, MRVM, 18859, Vieira, Piffort,…
CNTN1 transfected Lec1 cells Trasfection 20190805 Same protocol ICC HEK cells Samples: 15 healthy controls CNTN1 Blocking solution: rabbit serum 1:40 in PBS Patients’ sera…
CNTN1 transfected Lec1 cells Caspr1 transfected Lec1 cells CNTN1/ Caspr1 transfected Lec1 cells Trasfection 20190723-25 Same protocol ICC HEK cells Samples: MRVM 2011, 17595, negative…
2019.07.29 Morning: Prepare 4 plates of 60mm of diameter with 24 coverslips of 9mm. Treatment with laminin 2,5 microl/ml diluted in boratte buffer Incubate for…
CNTN1 transfected Lec1 cells Caspr1 transfected Lec1 cells CNTN1/ Caspr1 transfected Lec1 cells Trasfection date: 20190729-31 17595 16047 MRVM 2011 178859 19003 Vieira Piffort Regine…
2019.07.24 Morning: Prepare 3 plates of 60mm of diameter with 24 coverslips of 9mm. Treatment with Poly-D-Lysine (diluted 1/40 with boratte buffer) Incubate for 1h…