Objetivo: separar cels B, extraer RNA, hacer retrotranscripción y qPCR de cels B –> para establecer las condiciones óptimas de todo el proceso, y poder…
OBJECTIVES – To test whether stimulated B cells differentially express IL-10 upon IVIg treatment. The same samples used in the array will be used in…
OBJECTIVES – To test whether the new ODN vial recevived 20170123 properly stimulates B cells. We doubt its viability due to its temperature upon arrival,…
OBJECTIVES To extract RNA from IVIg treated and non-treated stimulated B cells from healthy individuals. INDIVIDUALS 4 CPT tubes from each of the following healthy…
OBJECTIVES – To test whether stimulated B cells differentially express EGR1 (as in the anergy paper by Seité et al.) IL-10 and/or Fcgr2b genes upon…
OBJECTIVES To extract RNA from IVIg treated and non-treated stimulated and non-stimulated B cells from healthy individuals. INDIVIDUALS 4 CPT tubes from two healthy controls…
OBJECTIVES – To test whether stimulated B cells differentially express IL-10 and/or Fcgr2b genes upon IVIg treatment. MATERIALS – High Capacity RNA-to-cDNA Kit (Ref:4387406, Applied…
OBJECTIVES To extract RNA from IVIg treated and non-treated stimulated B cells from healthy individuals. INDIVIDUALS 4 CPT tubes from each of the following healthy…
IL-10 production by non-stimulated and stimulated B cells will be assessed using the “IL-10 Secretion Assay – Detection Kit (PE), human ” kit by Miltenyi…
IL-10 production by non-stimulated and stimulated B cells will be assessed using the “IL-10 Secretion Assay – Detection Kit (PE), human ” kit by Miltenyi…