PROTOCOL ELISA anti-MAG TODOS LOS REACTIVOS VIENEN CON EL KIT
HiTrap IgM Purification HP – Cytiva Colums For 1 mL of serum sample Gradually add small amounts of solid ammonium sulphate to the sample until…
PROTOCOLO IGUAL QUE TEASING ixation: in acetone -20ºC for 10 min at RT Wash 2 times with PBS (5 min) with agitation Use the marker…
Preparation of ganglioside ELISA plates Reconstitute the ganglioside vials with 1 ml of chloroform/methanol (1:1), except in the case of GQ1b, which is reconstituted with…
PREPARATION: Fix the skin 30 min with 4% PFA in phosphate buffer (in eppendorf) Wash the skin in phosphate buffer (x 3, in eppendorf, without…
Conjugate the capture and the detection antibodies using the lightning-Link® Ulfa-Tag kit and Gold Conjugation Kit (40 nm, 20 OD) respectively, following the instructions below.…
EXTRACCIÓ DE PBMCs Extreure 4-5 tubs CPT. Centrifugar 20 minuts a 1650 g sense fre a temperatura ambient. Invertir els tubs per resuspendre les cèl·lules…
EXTRACCIÓ DE PBMCs Extreure 4-5 tubs CPT. Centrifugar 20 minuts a 1650 g sense fre a temperatura ambient. Invertir els tubs per resuspendre les cèl·lules…
MATERIALS: Bead reagent Detector reagent SBG reagent (Streptavidin-ß-galactosidase) RGP reagent (Resorufin ß-D-galactopyranoside) Calibrators Controls Sample diluent Quanterix SR-X™ Instrument Simoa Microplate Washer Simoa Plate Shaker…
MATERIALS: ELISA plates NUNC ref: 442404 Thermoscientific ELISA plate ref. 1581 Coating buffer: sodium carbonate (pH 9.6) Recombinant Human NF155 protein MAH-HRP IgG4 secondary antibody REF:…