PROTOCOL Pierce Classic Magnetic IP Kit 1.incubate serum 1h at 37ªC, in 4 plates con DRG neurons. Serum dilution 1/100. 20ul of serum in 2ml…
Protocol using Pierce Classic Magnetic IP Kit 1.incubate serum 1h at 37ªC, in 5 plates con DRG neurons. Serum dilution 1/100. 20ul of serum in…
OBJECTIVE: To test myelinated fibers IHC and nodo-paranodes in foot pads from a rat. We will perform 3 different conditions: MBP + caspr, MBP +…
OBJECTIVE: To test myelinated fibers IHC in foot pads from a rat. We will perform 2 different conditions for MBP + PGP and for Caspr…
OBJECTIVE: To test skin biopsy procedure in foot pads from a rat. We will test 2 conditions for fixation: 4% PFA in PBS and 4%…
Objective: to test IgG reactivity against SH-SY5Y neuroblastoma derived neurons in patients iwht small fiber neuropathy ICC protocol: Fix diferenciated SH-SY5Y cells with 4% PFA…
2019.04.25:seed cells (in proliferation medium) Coating with laminin 2’5 µg/ml diluted in Borate Buffer (for 1 h at 37ºC) Wash 2-3 times with PBS Seed aprox…
2019.01.11: Coating and seeding Prepare 5 plates of 60 mm of diameter with 26 coverslips (9 mm) Treatment with laminin 1h (2’4 microg/ml diluted in…
OBJECTIVE: To test anti- PLEXIN-D1 antibodies in patients with small fiber neuropathy SAMPLES: Patients with small fiber neuropathy: IVIg 4,5,6,7,9,10,11,12,14,15,16,17,18,19,21,23-40,42,44,45,47,49,50,52,54,56,59 Healthy control ICC Patient’s serum diluted…
OBJECTIVE: To test anti- PLEXIN-D1 antibodies in patients with small fiber neuropathy SAMPLES Patients with DRG IgG reactivity: IVIg 53,55,57,58, negative control ICC Patient’s serum diluted…