OBJECTIVE: To test IgG positivity towards PLEXIN-D1 in NF155 IgG CIDP patients. SAMLES: IVIg 1, 2, 3 Control 1, 2 ICC: Non-transfeccted HEK 293 cells Blocking…
2019.01.02 Morning: Prepare 3 plates of 60mm of diameter with 24 coverslips of 9mm. Treatment with Poly-D-Lysine (diluted 1/40 with boratte buffer) Incubate for 1h…
OBJECTIVE: To test anti- PLEXIN-D1 antibodies in patients with small fiber neuropathy SAMPLES Patients with DRG IgG reactivity: IVIg 2, 3, 8, 13, 20, 22, 41,…
27.11.18 TRANSFORMATION Morning: Preparation of LB agar plates with ampicillin 1/1000 Afternoon: E.Coli transformation –> Stratagene protocol: XL10-Gold Ultracompetent cells. Cat nº: 200314 28.12.18 STARTER Morning: …
OBJECTIVE: To test the expression of PLEXIN-D1 in HEK 293 cells Day 1: Prepare one plates of 60mm of diameter with 14 coverslips of 12mm. Treatment…
OBJECTIVE: To test the expression of PLEXIN-D1 in NON-TRANSFECTED NEUROBLASTOMA SH-SY5Y CELLS 18.12.10 COATING: Prepare 1 plates of 60mm of diameter with 14 coverslips of 12mm. Treatment…
2018.12.11 Prepare one plates of 60mm of diameter with 14 coverslips of 12mm. Treatment with Poly-D-Lysine (diluted 1/40 with boratte buffer) Incubate for 1h (37ºC,…
OBJECTIVE: To test IgG positivity towards PLEXIN D1 in small fiber neuropathy patients. SAMPLES: IVIg 1, 2 and 3, control 1 and 2, Ab anti-plexin D1…
Samples: 60 small fiber neuropathies from Maastrich (name: IVIg and a number) + positive control + 4 negative controls ( IgG) DRG NEURONS ICC Incubate with…