Samples: 60 small fiber neuropathies from Maastrich (name: IVIg and a number) + positive control + 4 negative controls ( IgG)
DRG NEURONS ICC
- Incubate with patient’s sera in a 24 wells plate: 1 hour 37ºC (prepare the dilution in the wells and then put one coverslip per well)
- For IgG: 2 µl of sera in 300 µl of NG medium
- For IgG: 2 µl of sera in 300 µl of NG medium
- Fix with PFA 4 % –> 10 min at room temperature
- Wash 1 time with PBS1x
- Incubate with secondary antibody (1 hour at room temperatura) –> GAH 488 IGg 1:1500 diluted in Goat serum 5%
- Wash 1 time with PBS1x
- Mount with Vectashield
RESULTS
Id | IgG/IgM | Result |
---|---|---|
IVIg1 | IgG | - |
IVIg2 | IgG | + |
IVIg3 | IgG | + |
IVIg4 | IgG | - |
IVIg5 | IgG | - |
IVIg6 | IgG | - |
IVIg7 | IgG | - |
IVIg8 | IgG | + |
IVIg9 | IgG | - |
IVIg10 | IgG | - |
IVIg11 | IgG | - |
IVIg12 | IgG | - |
IVIg13 | IgG | ++ |
IVIg14 | IgG | - |
IVIg15 | IgG | - |
IVIg16 | IgG | - |
IVIg17 | IgG | - |
IVIg18 | IgG | - |
IVIg19 | IgG | - |
IVIg20 | IgG | + |
IVIg21 | IgG | - |
IVIg22 | IgG | + |
IVIg23 | IgG | - |
IVIg24 | IgG | - |
IVIg25 | IgG | - |
IVIg26 | IgG | - |
IVIg27 | IgG | - |
IVIg28 | IgG | - |
IVIg29 | IgG | - |
IVIg30 | IgG | - |
IVIg31 | IgG | - |
IVIg32 | IgG | - |
IVIg33 | IgG | - |
IVIg34 | IgG | - |
IVIg35 | IgG | - |
IVIg36 | IgG | - |
IVIg37 | IgG | - |
IVIg38 | IgG | - |
IVIg39 | IgG | - |
IVIg40 | IgG | - |
IVIg41 | IgG | ++ |
IVIg42 | IgG | - |
IVIg43 | IgG | + |
IVIg44 | IgG | - |
IVIg45 | IgG | - |
IVIg46 | IgG | + |
IVIg47 | IgG | - |
IVIg48 | IgG | + |
IVIg49 | IgG | - |
IVIg50 | IgG | - |
IVIg51 | IgG | + |
IVIg52 | IgG | - |
IVIg53 | IgG | + |
IVIg54 | IgG | - |
IVIg55 | IgG | + |
IVIg56 | IgG | - |
IVIg57 | IgG | + |
IVIg58 | IgG | + |
IVIg59 | IgG | - |
IVIg60 | IgG | ++ |
Positive control CNTN1+ (15-198) | IgG | +++ |
Healthy control (man 29y) | IgG | - |
Healthy control (woman 23y) | IgG | - |
Healthy control (man 36y) | IgG | - |
Healthy control (woman 22y) | IgG | - |