Objetivo: separar cels B, extraer RNA, hacer retrotranscripción y qPCR de cels B –> para establecer las condiciones óptimas de todo el proceso, y poder…
Samples: Serum/antibody used Secondary Ab Result 15-198 (CNTN1+ patient) 1/100 GAH 488 IgG + + + CIDP 44 (CNTN1+ patient) 1/100 GAH 488 IgG +…
Protocol: Fixation: in acetone -20ºC for 10 min at RT Wash 2 times with PBS (5 min) with agitation Use the marker (DakoPen) to highlight…
Protocol: Fixation: in acetone -20ºC for 10 min at RT Wash 2 times with PBS (5 min) with agitation Use the marker (DakoPen) to highlight…
Samples: CIDP Rome: 19040, 19041,19042, 19043 Ab Screening CIDP: 18-531, 18-532, 18-534, 18-536, 18-553, 18-549, 18-551 CNTN1 positive control: 15-198 Protocol: Fixation: in acetone -20ºC…
Objective: to test a new GAH594 IgG antibody (109-585-003, Jackson) CNTN1-CASPR1: Blocking solution: 5% goat serum in PBS. Patient’s sera diluted 1/100 with 5% goat…
25.07.2018: NF155 Transfection The day before, the cells were seeded at 3 milion cells/plate (100 mm of diameter) For every plate of 100 mm…
Patients: Positive control (kit) Negative control (kit) 17-595 –> O’Nions 15-198 –> CNTN1 positive 15-195–> NF155 positive IHC: Comercial tissue sample: FA 1120-1005 (Euroimmun) Patient’s…
2018.06.04: Coating and seeding Prepare 4 plates of 35 mm of diameter with 5 coverslips (12 mm) Treatment with: Poly-L-Lysine 1h (0’1 mg/ml diluted in Borate…
2018.05.28: Coating and seeding Prepare 2 plates of 60mm of diameter with 14 coverslips (12 mm) Treatment with Poly-D-Lysine (diluted 1/40 with PBS) Incubate for…