Samples:
- IgG:
- unit patients
- controls (healthy, ELA and dysferlin patients)
- IgM:
- unit patients
- Zika (Willison)
- controls (healthy, ELA and dysferlin patients)
DRG NEURONS ICC
- Incubate with patient’s sera in a 24 wells plate: 1 hour 37ºC (prepare the dilution in the wells and then put one coverslip per well)
- For IgG: 2 µl of sera in 300 µl of NG medium
- For IgM: 7,5 µl of sera in 300 µl of NG medium
- For IgG: 2 µl of sera in 300 µl of NG medium
- Fix with PFA 4 % –> 10 min at room temperature
- Wash 1 time with PBS1x
- Incubate with secondary antibody (1 hour at RT) –> GAH 488 IGg/IgM 1:1500 diluted in Goat serum 5%
- Wash 1 time with PBS1x
- Mount with Vectashield
RESULTS
Name / pathology | Id | IgG/IgM | Result |
---|---|---|---|
M.Mir Barahona | 18-611 (ICQ) | IgG | - |
S.Barbora | 18-648 (ICQ) | IgG | + |
G. Muñoz García | 18-650 (ICQ) | IgG | - |
J.A.Pérez | 18-848 (ICQ) | IgG | + |
M. Cots Cusine | 18-849 (ICQ) | IgG | - |
Abel Roca Comas | 17-883 (ser.imm.) | IgG | - |
healthy control (woman 63y) | IgG | - | |
healthy control (woman 50y) | IgG | - | |
healthy control (woman 68y) | IgG | - | |
healthy control (man 69y) | IgG | - | |
healthy control (woman 57y) | IgG | - | |
healthy control (man 50y) | IgG | - | |
healthy control (man 80y) | IgG | - | |
healthy control (man 28y) | IgG | - | |
healthy control (woman 69y) | IgG | - | |
healthy control (man 29y) | IgG | ++ | |
ELA | 205-16 (ser. gen.) | IgG | - |
ELA | 206-03 (ser. gen.) | IgG | - |
ELA | 206-04 (ser. gen.) | IgG | - |
ELA | 206-05 (ser. gen.) | IgG | - |
ELA | 207-05 (ser. gen.) | IgG | + |
ELA | 207-06 (ser. gen.) | IgG | - |
Dysferlinophathy | cos 145 | IgG | + |
Dysferlinophathy | cos 146 | IgG | ++ |
Dysferlinophathy | cos 149 | IgG | - |
Dysferlinophathy | cos 150 | IgG | - |
Dysferlinophathy | cos 151 | IgG | - |
Dysferlinophathy | cos 152 | IgG | - |
Dysferlinophathy | cos 153 | IgG | ++ |
Dysferlinophathy | PDGF 25 | IgG | - |
Positive control (CNTN1+) | 15-198 | IgG | +++ |
Zika (Willison) | 3 | IgM | + |
Zika (Willison) | 4 | IgM | ++ |
Zika (Willison) | 5 | IgM | - |
Zika (Willison) | 6 | IgM | + |
Zika (Willison) | 8 | IgM | + |
Zika (Willison) | 10 | IgM | - |
Zika (Willison) | 11 | IgM | - |
Zika (Willison) | 13 | IgM | - |
Zika (Willison) | 21 | IgM | - |
Zika (Willison) | 24 | IgM | ++ |
Zika (Willison) | 25 | IgM | + |
M.Mir Barahona | 18-611 (ICQ) | IgM | - |
S.Barbora | 18-648 (ICQ) | IgM | - |
G. Muñoz García | 18-650 (ICQ) | IgM | - |
J.A.Pérez | 18-848 (ICQ) | IgM | - |
M. Cots Cusine | 18-849 (ICQ) | IgM | - |
Abel Roca Comas | 17-883 (ser.imm.) | IgM | + |
healthy control (woman 63y) | IgM | - | |
healthy control (woman 50y) | IgM | - | |
healthy control (woman 68y) | IgM | - | |
healthy control (man 69y) | IgM | - | |
healthy control (woman 57y) | IgM | - | |
healthy control (man 50y) | IgM | - | |
healthy control (man 80y) | IgM | - | |
healthy control (man 28y) | IgM | - | |
healthy control (woman 69y) | IgM | - | |
healthy control (man 29y) | IgM | - | |
ELA | 205-16 (ser. gen.) | IgM | - |
ELA | 206-03 (ser. gen.) | IgM | - |
ELA | 206-04 (ser. gen.) | IgM | - |
ELA | 206-05 (ser. gen.) | IgM | - |
ELA | 207-05 (ser. gen.) | IgM | - |
ELA | 207-06 (ser. gen.) | IgM | - |
Dysferlinophathy | cos 145 | IgM | - |
Dysferlinophathy | cos 146 | IgM | - |
Dysferlinophathy | cos 149 | IgM | - |
Dysferlinophathy | cos 150 | IgM | - |
Dysferlinophathy | cos 151 | IgM | - |
Dysferlinophathy | cos 152 | IgM | - |
Dysferlinophathy | cos 153 | IgM | ++ |
Positive control (anti-MAG+) | 09-6019 (Pérez Pulido) | IgM | - |