Descongelo 1 vial de células de Schwann humanas (línea comercial 1700, ScienceCell) en 1 placa de 100mm con gelatina 0,15%
Congelo 4 viales de p4 con 1 millón de cels/vial: en medio proliferación CS humanas + 10%DMSO
Congelo 5 viales de p6 con 1,3 millones de cels/vial : en medio proliferación CS rata + 10%DMSO
Sueros dilución 1/100 IgG + 1/50 IgM MUESTRAS MUESTRA SC IgG SC IgM DRG IgG DRG IgM C NEGATIVO NEG NEG NV(repetir) NV (repetir) CIDP…
PROTOCOLO DAY 1. PLANTAR 100.000 Cells/pocillo (culture slides) tras coating de 2 horas con polyD DAY 2. Trasfección según protocolo habitual DAY 3. Después de…
Primary culture Schwann cell IgG and IgM ICC is performed to test controls staining pattern. Samples: 2 sera from healthy controls, 5 sera from ALS…
Primary culture Schwann cell IgG and IgM ICC is performed to test controls staining pattern. Samples: 12 sera from controls, and 2 positive controls (Zika-GBS…
Primary culture Schwann cell IgG and IgM ICC is performed to test Zika-GBS patients staining pattern. Samples: 11 sera from Zika-GBS (Willison cohort), and 2…
Primary culture Schwann cell IgG and IgM ICC is performed to test Zika-GBS patients staining pattern. Samples: 11 sera from Zika-GBS (Willison cohort), and a…
04/10/2018 One vial of Schwann cells (primary culture) is thawed in 1 T75 flask coated with gelatin 0,15 % Growing medium: Schwann cell medium (commercial,…