Al día siguiente: *Al permeabilizar sin fijar se me han despegado todas las células y al aspirar el tritón las he perdido todas. REPETIR FIJANDO…
*También preparo una placa 6w de NF155 para comprobar que la inmunoabsorción funciona correctamente. *Marta hace inmunoabs de LIF y de NF155 el día 14.02.23…
OBJECTIVE: To test Caspr1 reactivity after serum pre-absorption with HEK cells transfected with CNTN1 or Caspr1 or CNTN1-Caspr1 contrasfection MATERIALS: ELISA plates NUNC ref: 442404…
Fixation: in acetone -20ºC for 10 min at RT Wash 2 times with PBS (5 min) with agitation Use the marker (DakoPen) to highlight the…
Objective: to do an immunoabsorption of CNTN1/Caspr1 in sera from 1 patient with antibodies anti-CNTN1 (Vieira 1:1000) 12.08.2019: Coating HEK293 Prepare 4 6-well plates Treatment with…
Objective: to do an immunoabsorption of CNTN1/Caspr1 in sera from 1 patient with antibodies anti-CNTN1 (18859 1:1000) 07.08.2019: Coating HEK293 Prepare 4 6-well plates Treatment with…
Objective: to test reactivity agains CNTN1/Caspr1 transfected HEK cells in PREABSORBED SERUM from patients 17595 and MRVM 1:800 (immunoabsorptions 24-26 and 29-31/7/19) Protocol: CNTN1/Caspr1 transfected HEK…
Objective: to do an immunoabsorption of CNTN1/Caspr1 in sera from 1 patient with antibodies anti-CNTN1/Caspr1 (MRVM) 29.07.2019: Coating HEK293 Prepare 4 6-well plates Treatment with Poly-D-Lysine…
Objective: to do an immunoabsorption of CNTN1/Caspr1 in sera from 1 patient with antibodies anti-CNTN1/Caspr1 (17595) 18.06.2019: Coating HEK293 Prepare 4 6-well plates Treatment with Poly-D-Lysine…
Objective: to do an immunoabsorption of CNTN1/Caspr1 in sera from 1 patient with antibodies anti-CNTN1/Caspr1 11.06.2019: Coating HEK293 Prepare 4 6-well plates Treatment with Poly-D-Lysine (diluted…