Muestras: Protocolo: Analizar resultados al microscopio de fluorescencia. Resultado –>Todas las muestras tienen un marcaje inespecífico con IgM. Repetiremos la ICQ con células vivas esta…
6 culture slides: doble CNTN1/CASPR1 *El día anterior se hace coating de Poly-D-lys 1/40 Protocolo:
2 culture slides perfil: hago una prueba con Lipofectamina 3000 que tenemos en la nevera (aunque caducada desde 2020). *Se hizo coating con poly-D-lys 1/40…
Day 1- 2021.03.22: Prepare 12 Corning® BioCoat™ 8-well culture slides à 354632 Corning. Coating with 0,3ml of Poly-D-Lysina (diluted 1/40 with sterile PBS x1) Incubate…
Day 1- 2021.03.10: Prepare 6 Corning® BioCoat™ 8-well culture slides à 354632 Corning. Coating with 0,3ml of Poly-D-Lysina (diluted 1/40 with sterile PBS x1) Incubate…
Day 1- 2021.03.10: Prepare 6 Corning® BioCoat™ 8-well culture slides à 354632 Corning. Coating with 0,3ml of Poly-D-Lysina (diluted 1/40 with sterile PBS x1) Incubate…
Day 1- 2021.03.08: Prepare 6 Corning® BioCoat™ 8-well culture slides à 354632 Corning. Coating with 0,3ml of Poly-D-Lysina (diluted 1/40 with sterile PBS x1) Incubate…
2019.01.02 Morning: Prepare 3 plates of 60mm of diameter with 24 coverslips of 9mm. Treatment with Poly-D-Lysine (diluted 1/40 with boratte buffer) Incubate for 1h…
2018.12.19: Prepare: 1 60mm-plate with 26 coverslips of 9mm. 2 100mm-plates (without coverslips) Treatment with Poly-D-Lysine (diluted 1/40 with boratte buffer) Incubate for 1h (37ºC,…
2018.12.11 Prepare one plates of 60mm of diameter with 14 coverslips of 12mm. Treatment with Poly-D-Lysine (diluted 1/40 with boratte buffer) Incubate for 1h (37ºC,…