PROTOCOLO Mismo PROTOCOLO IP, pero en este caso utilizamos magnetic beads para cadenas Kappa Pierce™ Protein L Magnetic BeadsNúmero de catálogo: 88849 IP en HEKS Transfectadas…
PROTOCOLO CBA NF155 and CNTN1 Bloqueo goat 5% 1h Serum 1/100 1h ANTICUERPOS SECUNDARIOS KAPPA Y LAMBDA BIOTINILADOS 1/500 1h ANTICUERPOS GOAT STREPTAVIDINA 594 32356…
rotocol using Pierce Classic Magnetic IP Kit 1.incubate serum 1h at 37ªC, in 5 plates con DRG neurons. Serum dilution 1/100. 30ul of serum in…
Protocol using Pierce Classic Magnetic IP Kit 1.incubate serum 1h at 37ªC, in 5 plates con DRG neurons. Serum dilution 1/100. 30ul of serum in…
Protocol using Pierce Classic Magnetic IP Kit 1.incubate serum 1h at 37ªC, in 5 plates con DRG neurons. Serum dilution 1/100. 20ul of serum in…
Protocol using Pierce Classic Magnetic IP Kit 1.incubate serum 1h at 37ªC, in 5 plates con DRG neurons. Serum dilution 1/100. 20ul of serum in…
Cotransfectamos HEKs con CNTN1+Caspr1 3 placas de 100/paciente Dia 1. Coating con lipofectamina y plantamos 3×10*6 cel Dia 2. transfección con 13,4ug DNA + 417ul…
Protocol using Pierce Classic Magnetic IP Kit 1.incubate serum 1h at 37ªC, in 5 plates con DRG neurons. Serum dilution 1/100. 20ul of serum in…
Samples: 17-219 (2000pg/mL of sera CNTN1 detected by ELISA in Amsterdam – Dr. Luuk Wieske) plasma from a CNTN1+ CIDP patient (no detectable CNTN1 in…
Objective: To detect serum CNTN1 in a CIDP patient. Sera CNTN1 is detected by ELISA but we do not have any confirmatory technique. We have…