OBJECTIVE: To test anti- PLEXIN-D1 antibodies in patients with small fiber neuropathy
SAMPLES
- Patients with DRG IgG reactivity: IVIg 2, 3, 8, 13, 20, 22, 41, 43, 46, 48, 51, 60.
- Patient with no DRG reactivity: IVIg 1
- Healthy control
ICC
- Patient’s serum diluted 1/100 in Blocking solution (rabbit serum 1/40) 1h
- Primary antibody anti-PLEXIN-D1 diluted 1/1000 (ab28762, Goat polyclonal) 1h
- Secondary antibodies: RAG488 (1/1000), RAH 594 (1/1000) 1h
- Vectashield mounting medium
RESULTS
All samples were negative
