OBJECTIVE: To test anti- PLEXIN-D1 antibodies in patients with small fiber neuropathy
SAMPLES
- Patients with DRG IgG reactivity: IVIg 53,55,57,58, negative control
ICC
- Patient’s serum diluted 1/100 in Blocking solution (rabbit serum 1/40) 1h
- Primary antibody anti-PLEXIN-D1 diluted 1/1000 (ab28762, Goat polyclonal) 1h
- Secondary antibodies: RAG488 (1/1000), RAH 594 (1/1000) 1h
- Vectashield mounting medium
RESULTS
All samples were negative