OBJECTIVE: To test anti- PLEXIN-D1 antibodies in patients with small fiber neuropathy
SAMPLES:
- Patients with small fiber neuropathy: IVIg 4,5,6,7,9,10,11,12,14,15,16,17,18,19,21,23-40,42,44,45,47,49,50,52,54,56,59
- Healthy control
ICC
- Patient’s serum diluted 1/100 in Blocking solution (rabbit serum 1/40) 1h
- Primary antibody anti-PLEXIN-D1 diluted 1/1000 (ab28762, Goat polyclonal) 1h
- Secondary antibodies: RAG488 (1/1000), RAH 594 (1/1000) 1h
- Vectashield mounting medium
RESULTS: All samples were negative
