Objective: To test the sensibility of ICC vs ELISA in detecting the serum positivity in NF155+ CIDP patients. We are going to do the ICC of the samples and we are going to use the ELISA results from the experiment preformed on 28.12.2018.
Samples: 18-872, 18-874, 18-875; 18-882; 18-858, CTRL – 2, CTRL – 4.
NF155 ICC:
- Transfection date: 2018.12.04
- Blocking solution: 5% goat serum in PBS.
- Patient’s sera diluted 1/100 for IgG with 5% goat serum in PBS. Make serial dilutions 1:3 of the samples in 5% goat serum.
- Primary antibody: AF3235 diluted 1/1000.
- Secondary antibodies: GAC488 + GAH 594 IgG Jackson (1/1000).
- Vectashield mounting medium
RESULTS:
The sensibility did not vary between the techniques.
The results are represented here.
