MATERIALS: ELISA plates NUNC ref: 442404 Thermoscientific Coating buffer: sodium carbonate (pH 9.6) Recombinant Human NF155 protein RAH-HRP IgG secondary antibody, Dako P0214 (For total…
DC Protein Assay – BioRad- Dilute BSA to 4 mg/ml –> stock -20ºC at 10mg/ml (16ul BSA + 24 ul RIPA) Prepare the dilutions of…
Anti-CNTN1: R&D Systems, AF904 Anti-NF: R&D Systems, AF3235 Anti-CASPR1: ABCAM, ab133634 Anti-CASPR2: ABCAM, ab33994 Goat anti-chicken 488: ThermoFisher, A-11039 Goat anti-rabbit 488: ThermoFisher, A-11008 Rabbit-anti-goat…
Use precast gels from BioRad (10 wells, 4-15 %) Warm the samples at 96ºC for 5 minutes Load samples in the wells (30 ul per…
Pierce Magnetic IP Kit Incubate the culture or the tissue with the serum of the patient to be analyzed (for IgG 1/100): If the IP…
Day 0:seed cells (in proliferation medium) Coating with laminin 2’5 µg/ml diluted in Borate Buffer (for 1 h at 37ºC) Wash 2-3 times with PBS…
CNTN1 ICC: Blocking solution: Rabbit serum diluted 1/40 in PBS. Patient’s sera diluted 1/100 Primary antibody (anti-CNTN1 AF904) diluted 1/1000. Secondary antibodies: RAG 488 +…
Put the nerve between wet gauzes with PBS on a Petri dish, and put it on ice. Clean the nerve by removing the fat, connective…
Fixation: in acetone -20ºC for 10 min at RT Wash 2 times with PBS (5 min) with agitation Use the marker (DakoPen) to highlight the…