CNTN1 ICC:
- Blocking solution: Rabbit serum diluted 1/40 in PBS.
- Patient’s sera diluted 1/100
- Primary antibody (anti-CNTN1 AF904) diluted 1/1000.
- Secondary antibodies: RAG 488 + RAH 594 IgG (1/1000)
- Vectashield mounting medium.
NF140, NF155, NF186 ICC:
- Blocking solution: Goat serum 5 % diluted in PBS.
- Patient’s sera diluted 1/100
- Primary antibody (anti-NF AF3235) diluted 1/1000.
- Secondary antibodies: GAC 488 + GAH 594 IgG (1/1000)
- Vectashield mounting medium.
Double CNTN1-CASPR1 ICC:
- Blocking solution: Goat serum 5 % diluted in PBS.
- Patient’s sera diluted 1/100
- Primary antibody (anti-CASPR1) diluted 1/250.
- Secondary antibodies: GAR 488 + GAH 594 IgG (1/1000)
- Vectashield mounting medium.
