- CNTN1 transfected Lec1 cells
Trasfection 20190805
Same protocol ICC HEK cells
Samples: 15 healthy controls
CNTN1
- Blocking solution: rabbit serum 1:40 in PBS
- Patients’ sera diluted 1:100 (rabbit serum 1:40 in PBS)
- Ac anti-CNTN1 diluted 1/1000
- RAH 594 IgG and RAG 488 IgG diluted 1/1000
- Vectashield mounting medium
RESULTS:
There is a background in almost all samples, while the positive control is clearly positive. I think there is a channel crossing
