SAMPLES: 18859, Vieira, Piffrei, Regine, negative control, CNTN1+ (15198)
CNTN1:
- Blocking solution: rabbit serum 1:40 in PBS
- Patients’ sera diluted 1:100 (rabbit serum 1:40 in PBS)
- Ac anti-CNTN1 diluted 1/1000
- RAH 594 IgG and RAG 488 IgG diluted 1/1000
- Vectashield mounting medium
Caspr 1 and CNTN1/Caspr1: :
- Blocking solution: goat 5% in PBS
- Patients’ sera diluted 1:100 ( goat 5% in PBS)
- Ac anti-Caspr1 diluted 1/250
- GAH 594 IgG and GAR 488 IgG diluted 1/1000
- Vectashield mounting medium
RESULTS:
Patient 18595 seems positive doubtful because it has a lot of background and membrane marking is not very evident
| SAMPLE | CNTN1 | Caspr1 | CNTN1/Caspr1 (goat) |
|---|---|---|---|
| 18859 | POSITIVE?? | NEGATIVE | POSITIVE, no colocalization |
| Vieira | NEGATIVE | NEGATIVE | POSITIVE, no colocalization |
| Piffort | NEGATIVE | POSITIVE | POSITIVE, no colocalization |
| Regine | NEGATIVE | REPEAT | POSITIVE, no colocalization |
| Negative control | NEGATIVE | NEGATIVE | NEGATIVE |
| CNTN1+ (15198) | POSITIVE ++ | NEGATIVE | POSITIVE, no colocalization |
