OBJECTIVE: to test CNTN1 and Caspr1 expression in Lec1 cells
A) Caspr1 transfected Lec1 cells:
TO TEST CNTN1 expression:
- Blocking solution: rabbit serum 1:40 in PBS
- Ac anti-CNTN1 diluted at 1/1000, 1/500, 1/250
- RAG 488 IgG diluted 1/1000
- Vectashield mounting medium
RESULTS: NEGATIVE
B) Non trasnfected Lec1 cells:
TO TEST CNTN1 expression:
- Blocking solution: rabbit serum 1:40 in PBS
- Ac anti-CNTN1 diluted at 1/500, 1/250
- RAG 488 IgG diluted 1/1000
- Vectashield mounting medium
RESULTS: NEGATIVE AT 1/500, VERY VERY MILD POSITIVITY AT 1/250
TO TEST Caspr1 expression:
- Blocking solution: goat 5% in PBS
- Ac anti-Caspr1 diluted 1/250GAR 488 IgG diluted 1/1000
- Vectashield mounting medium
RESULTS: VERY MILD DOTTED CITOPLASMATIC STAINNING
C) CNTN1 transfected Lec1 cells:
TO TEST Caspr1 expression:
- Blocking solution: goat 5% in PBS
- Ac anti-Caspr1 diluted 1/250GAR 488 IgG diluted 1/1000
- Vectashield mounting medium
RESULTS: NEGATIVE
