a) CNTN1/Caspr1 co-transfected HEK (Caspr1 mAb)
- Blocking solution: goat 5%
- Patients’ sera diluted 1:100
- Ac anti-Caspr1 diluted 1/250
- GAH 594 IgG and GAR 488 IgG diluted 1/1000
- Vectashield mounting medium
RESULTS
- negative control: negative
- 19204: positive
- 19209: positive
- 19212: positive
- 19216: positive
b) Caspr1 transfected HEK (Caspr1 mAb)
- Blocking solution: goat 5%
- Patients’ sera diluted 1:100
- Ac anti-Caspr1 diluted 1/250
- GAH 594 IgG and GAR 488 IgG diluted 1/1000
- Vectashield mounting medium
RESULTS
- negative control: negative
- 19204: positive
- 19209: positive
- 19212: mild positive
- 19216: mild positive
c) CNTN1/Caspr1 co-transfected Lec1 (Caspr1 mAb)
- Blocking solution: goat 5%
- Patients’ sera diluted 1:100
- Ac anti-Caspr1 diluted 1/250
- GAH 594 IgG and GAR 488 IgG diluted 1/1000
- Vectashield mounting medium
RESULTS
- negative control: negative
- 19204: positive
- 19209: positive
- 19212: positive
- 19216: positive
d) Caspr1 transfected Lec1 (Caspr1 mAb)
- Blocking solution: goat 5%
- Patients’ sera diluted 1:100
- Ac anti-Caspr1 diluted 1/250
- GAH 594 IgG and GAR 488 IgG diluted 1/1000
- Vectashield mounting medium
RESULTS
- negative control: negative
- 19204: positive
- 19209: positive
- 19212: mild positive
- 19216: mild positive
e) CNTN1/Caspr1 co-transfected Lec1 (CNTN1 mAb)
- Blocking solution: rabbit serum 1:40 in PBS
- Patients’ sera diluted 1:100 (rabbit serum 1:40 in PBS)
- Ac anti-CNTN1 diluted 1/1000
- RAH 594 IgG and RAG 488 IgG diluted 1/1000
- Vectashield mounting medium
RESULTS
- negative control: negative
- 19204: positive
- 19209: positive
- 19212: positive
- 19216: positive
