a) CNTN1/Caspr1 co-transfected HEK
- Blocking solution: goat 5%
- Patients’ sera diluted 1:100
- Ac anti-Caspr1 diluted 1/250
- GAH 594 IgG and GAR 488 IgG diluted 1/1000
- Vectashield mounting medium
RESULTS
- negative control: negative
- Vieira: positive
- 19186: positive
b) Caspr1-transfected HEK
- Blocking solution: goat 5%
- Patients’ sera diluted 1:100
- Ac anti-Caspr1 diluted 1/250
- GAH 594 IgG and GAR 488 IgG diluted 1/1000
- Vectashield mounting medium
RESULTS
- negative control: negative
- 16047: negative (FOTO)
- 19186: negative
- Piffort (positive control) ++
c) CNTN1/Caspr1 co-transfected Lec1 (Caspr mAb)
- Blocking solution: goat 5%
- Patients’ sera diluted 1:100
- Ac anti-Caspr1 diluted 1/250
- GAH 594 IgG and GAR 488 IgG diluted 1/1000
- Vectashield mounting medium
RESULTS:
- negative control: negative
- 19186: positive ++, POOR COLOCALIZATION WITH CASPR1
d) CNTN1/Caspr1 co-transfected Lec1 (CNTN1 mAb)
- Blocking solution: rabbit serum 1:40 in PBS
- Patients’ sera diluted 1:100 (rabbit serum 1:40 in PBS)
- Ac anti-CNTN1 diluted 1/1000
- RAH 594 IgG and RAG 488 IgG diluted 1/1000
- Vectashield mounting medium
RESULTS:
- negative control: negative
- 19186: positive ++, MODERATE COLOCALIZATION WITH CNTN1
e) Caspr1 transfected Lec1
- Blocking solution: goat 5%
- Patients’ sera diluted 1:100
- Ac anti-Caspr1 diluted 1/250
- GAH 594 IgG and GAR 488 IgG diluted 1/1000
- Vectashield mounting medium
RESULTS:
- negative control: negative
- 19186: negative
- Piffort: positive ++, POOR COLOCALIZATION WITH CASPR1
f) CNTN1 transfected Lec1
- Blocking solution: rabbit serum 1:40 in PBS
- Patients’ sera diluted 1:100 (rabbit serum 1:40 in PBS)
- Ac anti-CNTN1 diluted 1/1000
- RAH 594 IgG and RAG 488 IgG diluted 1/1000
- Vectashield mounting medium
RESULTS:
- negative control: negative
- 19186: negative
- 15198 (CNTN1+): positive
