20200518: ICC FLOT1/2 kit Euroimmun (controls and positive samples)

SAMPLES:

• 1-8) MMN patients: 09-2582, 09-299, 09-1218, 09-1461, 09-292, 09-190, 09-254, 09-1804 (dil. 1/10)
• 9) MS 163 (dil. 1/10)
• 10) MS 402 (dil.1/10)
• 11) MS 405 (dil.1/10)
• 12) MS 406 (dil.1/10)
• 13) MS 158 (dil.1/50)
• 14) MS 163 (dil. 1/50)
• 15) MS 247 (dil.1/50)
• 16) MS 307 (dil.1/50)
• 17) MS 316 (dil.1/50)
• 18) MS 406 (dil.1/50)
• 19) MS 158 (dil.1/30)
• 20) MS 163 (dil.1/30)

PROCEDURE

• Patient’s sera diluted 1/10 (or 1/30 or 1 /50) in PBS-Tween 0,2% (LCR 1/5) –> add 30 microl to each reaction field in the reagent tray: place the biochip slides on them and incubate for 30 min at RT
• Rinse the biochips with a flush of PBS-Tween 0,2% and immerse them in a cuvette containing PBS-Tween 0,2% for 5 minutes.
• Add 25 microL of the fluorescein-labelled secondary antibody (kit) in each reaction field: place the biochip slides on them and incubate for 30 min at RT
• Rinse the biochips with a flush of PBS-Tween 0,2% and immerse them in a cuvette containing PBS-Tween 0,2% for 5 minutes.
• Mount with the mounting medium from the kit

RESULT

• 1-8) MMN patients: 09-2582, 09-299, 09-1218, 09-1461, 09-292, 09-190, 09-254, 09-1804 (dil. 1/10): todas las muestras –
• 9) MS 163 (dil. 1/10): +
• 10) MS 402 (dil.1/10): –
• 11) MS 405 (dil.1/10): –
• 12) MS 406 (dil.1/10): –
• 13) MS 158 (dil.1/50): +
• 14) MS 163 (dil. 1/50): + (weak)
• 15) MS 247 (dil.1/50): –
• 16) MS 307 (dil.1/50): + +
• 17) MS 316 (dil.1/50): + +
• 18) MS 406 (dil.1/50): –
• 19) MS 158 (dil.1/30): + +
• 20) MS 163 (dil.1/30): + (weak)