Objective: To test MS patients for IgG positivity towards FLOT1/FLOT2. Samples: 101-125 and CTRL – Transfection date: 2018.11.08 Blocking solution: 5% goat serum in PBS Patient’s…
2018.11.05 Prepare 6 plates of 60mm of diameter with 26 coverslips of 9mm. Treatment with Poly-D-Lysine (diluted 1/40 with boratte buffer) Incubate for 1h (37ºC,…
Objective: to test the doble transfection of FLOT1 AND FLOT2 (with the same methodology of the paper that describes these autoantibodies) 2018.10.15 Prepare 2 plates…
Objective: To test MS patients for IgG positivity towards FLOT1/FLOT2. Samples: 123-143, CTRL – Ana Blocking solution: 5% goat serum in PBS Patient’s sera diluted 1/100…
Objective: To test MS patients for IgG positivity towards FLOT1/FLOT2. Samples: 101-122, CTRL – Blocking solution: 5% goat serum in PBS Patient’s sera diluted 1/100 for…
Objective: to test doble transfection of flotillin 1 and flotillin 2. Blocking solution: 5% goat serum in PBS Comercial anti-flotillin1 dilluted 1/50 (1h) Secondary antibody: GAR488…
Objective: to test the doble transfection of flotillin 1 and flotillin 2. Day 1- 2018.09.03: Prepare 1 plates of 60mm of diameter with 24 coverslips…
Objective: to test the doble transfection of flotillin 1 and flotillin 2. Day 1- 2018.08.27: Prepare 1 plates of 60mm of diameter with 14 coverslips…
Objective: to test the doble transfection of FLOT1 AND FLOT2. Day 1- 2018.08.20: Prepare 4 plates of 60mm of diameter with 14 coverslips of 12mm.…