20210223: ICC FLOT1/2 kit Euroimmun (positive samples)

SAMPLES:

1. EM-158
2. EM-163
3. EM-390
4. EM-223
5. EM-316
6. EM-247
7. EM-307
8. EM-397
9. EM-352 (LCR)
10. EM-406

PROCEDURE: Kit Euroimmun (FA-1124-1005-50)

• Patient’s sera diluted 1/10 in PBS-Tween 0,2% (LCR 1/5) –> add 30 microl to each reaction field in the reagent tray: place the biochip slides on them and incubate for 30 min at RT
• Rinse the biochips with a flush of PBS-Tween 0,2% and immerse them in a cuvette containing PBS-Tween 0,2% for 5 minutes.
• Add 25 microL of the fluorescein-labelled secondary antibody (kit) in each reaction field: place the biochip slides on them and incubate for 30 min at RT
• Rinse the biochips with a flush of PBS-Tween 0,2% and immerse them in a cuvette containing PBS-Tween 0,2% for 5 minutes.
• Mount with the mounting medium from the kit

RESULT

1. EM-158 + +
2. EM-163 +
3. EM-390 no se ve nada
4. EM-223 + débil
5. EM-316 + + +
6. EM-247 +
7. EM-307 + +
8. EM-397 + + +
9. EM-352 (LCR) +
10. EM-406 –