SAMPLES:
- First group:
- 1-23) Miastenia Gravis patients: 190-11, 191-03, 192-04, 192-05, 193-17, 194-07, 194-09, 195-04, 195-15, 197-06, 198-09, 198-15, 199-08, 199-20, 201-07, 202-08, 203-03, 203-05, 203-06, 203-09, 203-17, 204-19, 219-04
- 24) GBS IGOS 19
- 25) Positive control MS 163
- Second group:
- GBS IGOS 20-24, 26-27
- CIDP 26-30
- CMT 26-31
- MMN: 09-3960, 09-200, 16-200, 15-611, 09-5162, 09-3200, 187-03
- Positive control MS 163
PROCEDURE
- Patient’s sera diluted 1/10 in PBS-Tween 0,2% (LCR 1/5) –> add 30 microl to each reaction field in the reagent tray: place the biochip slides on them and incubate for 30 min at RT
- Rinse the biochips with a flush of PBS-Tween 0,2% and immerse them in a cuvette containing PBS-Tween 0,2% for 5 minutes.
- Add 25 microL of the fluorescein-labelled secondary antibody (kit) in each reaction field: place the biochip slides on them and incubate for 30 min at RT
- Rinse the biochips with a flush of PBS-Tween 0,2% and immerse them in a cuvette containing PBS-Tween 0,2% for 5 minutes.
- Mount with the mounting medium from the kit
RESULT
All the samples were negative
