Objective: to do an immunoabsorption of CNTN1/Caspr1 in sera from 1 patient with antibodies anti-CNTN1 (Vieira 1:1000) 12.08.2019: Coating HEK293 Prepare 4 6-well plates Treatment with…
Objective: to do an immunoabsorption of CNTN1/Caspr1 in sera from 1 patient with antibodies anti-CNTN1 (18859 1:1000) 07.08.2019: Coating HEK293 Prepare 4 6-well plates Treatment with…
OBJECTIVE: to test CNTN1 and Caspr1 expression in Lec1 cells A) Caspr1 transfected Lec1 cells: TO TEST CNTN1 expression: Blocking solution: rabbit serum 1:40 in…
CNTN1 transfected Lec1 cells Trasfection 20190805 Same protocol ICC HEK cells (but without commercial Ab= Samples: CNTN1/Caspr1 positive patients: 17595, 16047, MRVM, 18859, Vieira, Piffort,…
CNTN1 transfected Lec1 cells Trasfection 20190805 Same protocol ICC HEK cells Samples: 15 healthy controls CNTN1 Blocking solution: rabbit serum 1:40 in PBS Patients’ sera…
SAMPLES: 18859, Vieira, Piffrei, Regine, negative control, CNTN1+ (15198) CNTN1: Blocking solution: rabbit serum 1:40 in PBS Patients’ sera diluted 1:100 (rabbit serum 1:40 in…
Objective: to test reactivity agains CNTN1/Caspr1 transfected HEK cells in PREABSORBED SERUM from patients 17595 and MRVM 1:800 (immunoabsorptions 24-26 and 29-31/7/19) Protocol: CNTN1/Caspr1 transfected HEK…
Objective: to test IgG reactivity against SH-SY5Y neuroblastoma derived neurons in CNTN1/Caspr1+ patients ICC protocol: Fix diferenciated SH-SY5Y cells with 4% PFA 5 min Wash…
Objective: to do an immunoabsorption of CNTN1/Caspr1 in sera from 1 patient with antibodies anti-CNTN1/Caspr1 (MRVM) 29.07.2019: Coating HEK293 Prepare 4 6-well plates Treatment with Poly-D-Lysine…
CNTN1 transfected Lec1 cells Caspr1 transfected Lec1 cells CNTN1/ Caspr1 transfected Lec1 cells Trasfection 20190723-25 Same protocol ICC HEK cells Samples: MRVM 2011, 17595, negative…