Coating and seeding: 22.05.18 [see the post 20180522 – 20180529: HTB-11 cells diferentiation (exp. 4) + ICC] ICC protocol: Fix with PFA 4% (aprox 5…
Day 0- 2018.05.22: Coating and seeding Prepare 2 plates of 60mm of diameter with 14 coverslips (12 mm) Treatment with Poly-D-Lysine (diluted 1/40 with PBS)…
Day 0- 2018.05.14: Coating and seeding Prepare plates of 60mm of diameter with 14 coverslips (12 mm) Treatment with Poly-D-Lysine (diluted 1/40 with PBS) Incubate…
Diferenciació HTB-11 a neurones RA 10 µM Medi amb 10 % FBS RA 5 µM + Chol 5 µg/ml Medi amb 10 % FBS NGF…
Diferenciació HTB-11 a neurones Dia 0 –> Sembrar cèl·lules Sembrar a placa de 24 pous 5000 cèls/cm2 (coating amb gelatina 0’15 %) Cada pou té 1’82…
2018.04.16: Prepare 4 plates of 60mm of diameter with 24 coverslips (9 mm) Treatment with Poly-D-Lysine (diluted 1/40 with PBS) Incubate for 1h (37ºC, 5%…
Day 1- 2018.02.14: Prepare 8 plates of 60mm of diameter with 24 coverslips of 9mm and 1 plate of 12 coverslips of 12mm. Treatment with…
Day 1- 2018.01.23: Prepare 7 plates of 60mm of diameter with 12 coverslips of 12mm. Treatment with Poly-D-Lysina (diluted 1/40 with PBS 1x) Incubate for 1h…
DNA Purification from Blood or Body Fluids OBJECTIVE To isolate DNA from a non-concentrted and a previously concentrated patient serum using the Qiamp DNA Blood…
Se reprecipita el DNA de la muestra de suero ct extraída el 23/01/2018, concretamente la muestra extraída a partir de 200 mcl de suero de…