Objective We’ll do the same experiment but with rat nerve instead of skin trying to verify the technique because we know how nerves looks like.…
Objective Set up IHC free-floating technique with glabrous rat skin. We’ll see neurofilament and try CASPR to detect nodes. Materials see rat skin IHC 3 Mouse-anti-CASPR Procedure…
Objective Set up IHC free-floating technique with glabrous rat skin. We’ll see neurofilament and MBP to detect myelin fibers. Materials see rat skin IHC 2…
Objective Set up a new technique, rat skin IHC, with changes respect 1: Change 1: filter secondary Ab Change 2: permeability cells with PBS-triton0,3% …
Objective Set up a new technique, rat skin IHC Materials Paraformaldehyde Sucrose 30% + azide 15mM / PBS 24-well plate PBS Cryostat (OTC, criomold) Goat…
OBJECTIVE To test 3 new patients with CIDP suspicion for anti-CNTN1 -NF155 antibodies MATERIALS NF155-HEK cell line, grown in coverslips and frozen (see previous experiment)…
OBJECTIVE To create a stable-transfectant that expresses NF155 constitutively MATERIALS – pCMV6-NF155-myc-DDK purchased from Origene. – Geneticin/G418 Life Technologies – Chicken polyclonal anti-NF – GAC…
Yesterday plasma exchange on NFASC 155+ patient Jaume Marcet Lucas. Stored O/N at 4ºc and today proceeded to aliquoting. We had: Around 600mL concentrated plasma…