20180416 – 20180417: Coating and freeze of HTB-11 coverslips

2018.04.16:

• Prepare 4 plates of 60mm of diameter with 24 coverslips (9 mm)
• Treatment with Poly-D-Lysine (diluted 1/40 with PBS)
• Incubate for 1h (37ºC, 5% CO2).
• Wash with PBS 1x (4 times)
• Trypsin-EDTA a plate of HEK293.
• For every plate: 5ml of HEK medium + the volume that correspond to 1×10⁶ of cells
• Incubate at 37ºC for 48h.

2018.04.17:

• Fix with PFA 4% . Incubation at RT during 10min.
• Wash with PBS 1x
• Blocking solution: 5% goat serum in PBS for 1h at RT.
• Remove the blocking solution and freeze at -80ºC