Objectives: To test CIDP patients for IgG positivity towards NF155, NF186 and NF140. Samples: NF155, NF186, NF140 ICC: HEKs 293 Cells Transfection: 2018.07.26 Blocking solution:…
Objectives: To test CIDP patients for IgG positivity towards NF155 and NF140. Samples: For NF155: For NF140: NF155, NF140 ICC: HEKs 293 Cells Transfection: 2018.01.16…
Objectives: To test patient 18-620 for IgG positivity towards CNTN1 and CNTN1/CASPR. Samples: 18-620 CNTN1 ICC: Blocking solution: Rabbit serum diluted 1/40 in PBS. Patient’s…
Coating and seeding: 28.05.18 [see the post 20180601: ICC HTB-11 neurons (different cell fixation methods) ] ICC protocol: Take 14 coverslips and put them in…
Objectives: To test if CNTN1 positive patients send from another hospitals are positive (samples to do HLA test). Patients: CNTN1 ICC: HEKs 293 Cells…
HTB-11 ICC: Samples: Patient’s sera diluted 1/100 with 5 % Goat Serum. Secondary antibody: GAH488 diluted 1/1000 Vectashield mounting medium Results: 102 + The…
HTB-11 ICC: Samples: Patient’s sera diluted 1/100 with 5 % Goat Serum. Secondary antibody: GAH488 diluted 1/1000 Vectashield mounting medium Results: 18-539 + 18-543 ++…
HTB-11 ICC: Samples: Patient’s sera diluted 1/100 with 5 % Goat Serum. Secondary antibody: GAH488 diluted 1/1000 Vectashield mounting medium Results: El paciente 18-517 es…
DRG NEURONS ICC Incubate with patient’s sera in a 24 wells plate: 1 hour 37ºC (prepare the dilution in the wells and then put one…
SAMPLES We will test the Zika samples from Dr. Willison’s lab and 4 samples from our ICQ database. ICQ samples: 18-612 Guillem Mateo Nieto 18-613…