Objectives: To test CIDP patients for IgG positivity towards NF155 and NF140.
Samples:
For NF155:
| CODE | RESULT |
|---|---|
| 46 | - |
| 47 | - |
| 48 | - |
| 49 | - |
| 50 | - |
| 51 | - |
| 52 | - |
| 53 | - |
| 54 | - |
| 55 | - |
| 56 | - |
| 57 | - |
| 58 | - |
| 59 | - |
| 60 | - |
| 61 | - |
| 62 | - |
| 63 | - |
| 64 | - |
| 65 | - |
| 66 | - |
| 67 | - |
| 68 | - |
| 69 | - |
| 70 | - |
| 71 | - |
| 72 | - |
| 73 | - |
| 74 | - |
| 75 | - |
| 76 | - |
| 77 | - |
| CTRL - | - |
| CTRL + | + |
For NF140:
| CODE | RESULT |
|---|---|
| 46 | - |
| 47 | - |
| 48 | - |
| 49 | - |
| 50 | - |
| 51 | - |
| 52 | - |
| 53 | - |
| 54 | - |
| 55 | - |
| 56 | - |
| 57 | - |
| 58 | - |
| 59 | - |
| 60 | - |
| 61 | - |
| 62 | - |
| 63 | - |
| 64 | - |
| 65 | - |
| 66 | - |
| 67 | - |
| CTRL - | - |
| CTRL + | + |
NF155, NF140 ICC:
- HEKs 293 Cells Transfection: 2018.01.16 /30.05.2018
- Blocking solution: 5% goat serum in PBS.
- Patient’s sera diluted 1/100 for IgG with 5% goat serum in PBS.
- Primary antibody: AF3235 diluted 1/1000.
- Secondary antibodies: GAC 488 (1/1000) + GAH 594 IgG (1/1000)
- Vectashield mounting medium.
Results: All samples were negative.
