Objectives: To test if CNTN1 positive patients send from another hospitals are positive (samples to do HLA test).
Patients:
| Mostres | IgG/IgM | Result |
|---|---|---|
| 18-656 | IgG | + |
| 18-660 | IgG | + |
| 18-661 | IgG | - |
| 18-662 | IgG | - |
| Plasma Clinica Mayo 43171 | IgG | - |
| Plasma Clinica Mayo 43172 | IgG | - |
| 214-09 (CMT) | IgG | - |
| Positive control 15-198 | IgG | + |
| Negative control | IgG | - |
CNTN1 ICC:
- HEKs 293 Cells Transfection: 16.01.2018
- Blocking solution: Rabbit serum diluted 1/40 in PBS.
- Patient’s sera diluted 1/100 for IgG with rabbit serum 1/40.
- Primary antibody: AF904 diluted 1/1000.
- Secondary antibodies: RAG 488 + RAH 594 IgG (1/1000)
- Vectashield mounting medium.
Results: see the table
- 2 of the 3 samples from Würzburg are negative towards CNTN1. Maybe this patients have been treated and the titles of CNTN1 are undetectable.
- Plasmas from Mayo Clinic are negative towards CNTN1
