Objectives: To test patient 18-620 for IgG positivity towards CNTN1 and CNTN1/CASPR.
Samples: 18-620
CNTN1 ICC:
- Blocking solution: Rabbit serum diluted 1/40 in PBS.
- Patient’s sera diluted 1/100 for IgG with rabbit serum 1/40.
- Primary antibody: AF904 diluted 1/1000.
- Secondary antibodies: RAG 488 + RAH 594 IgM (1/1000)
- Vectashield mounting medium.
CNTN1/CASPR ICC:
- Blocking solution: Goat serum diluted at 5% in PBS.
- Patient’s sera diluted 1/100 for IgG with goat serum 5% in PBS.
- Primary antibody: Ab 1336341 diluted 1/250
- Secondary antibodies: GAR488 + GAH 594 IgG (1/1000)
- Vectashield mounting medium.
RESULTS: The sample was negative for IgG towards CNTN1 and CNTN1/CASPR.
