DRG NEURONS ICC
- Incubate with patient’s sera in a 24 wells plate: 1 hour 37ºC (prepare the dilution in the wells and then put one coverslip per well)
- For IgG: 2 µl of sera in 300 µl of NG medium
- Fix with PFA 4 % –> 10 min at room temperature
- Wash 1 time with PBS1x
- Incubate with secondary antibody (1 hour at room temperatura) –> GAH 488 IGg 1:1500 diluted in Goat serum 5%
- Wash 1 time with PBS1x
- Mount with Vectashield
RESULTS
| Patient name | Sample ID | IgG/IgM | Result | |
|---|---|---|---|---|
| 1 | Pérez García, Margarita | 18-619 | IgG | - |
| 2 | Mir Barahona, Meritxell | 18-611 | IgG | - |
| 3 | Mateo Nieto, Guillem | 18-612 | IgG | - |
| 4 | Flores Arroyo, Jose Luis | 18-599 | IgG | - |
| 5 | Garreta Novell, Jose Mª | 214-03 | IgG | - |
| 6 | Vidondo Oses, Valentín | 214-04 | IgG | - |
| 7 | Serrano Andreu, Berta | 213-21 | IgG | + |
| 8 | Saline Perich, Marta | 213-10 | IgG | - |
| 9 | Colmenero Lázaro, Prudencio | 211-06 | IgG | - |
| 10 | Volpe Volpe, Rosa Mª | 201-04 | IgG | + |
| 11 | García Bonada, MªLourdes | 201-03 | IgG | - |
| 12 | Guix Alonso, M.J. | 201-02 | IgG | - |
| 13 | Segui Santana, Carmen | 200-12 | IgG | + |
| 14 | Vigas Salor, Xavier | 200-08 | IgG | - |
| 15 | Sánchez Pérez, MªIsabel | 199-16 | IgG | - |
| 16 | Morato Ferret, Ana Mª | 198-18 | IgG | - |
| 17 | Navarro Navarro, Francisco | 198-08 | IgG | - |
| 18 | Domingo Pardell, Ramon | 191-16 | IgG | + |
| 19 | Fernández Biarnau, J. | 191-12 | IgG | - |
| 20 | Martínez Fernández, Rosa Mª | 191-10 | IgG | - |
| 21 | Cussó, Pere | 18-600 (control +) | IgG | ++ |
| 22 | Control - (edu) | IgG | - | |
| 23 | Control - 11 | IgG | - |
