We use the product of the IP done on 14.01.2019 (JGV, C+, C-), to see if we can detect the CNTN1 in the positive control.…
2019.01.11: Coating and seeding Prepare 5 plates of 60 mm of diameter with 26 coverslips (9 mm) Treatment with laminin 1h (2’4 microg/ml diluted in…
2018.12.31: Coating and seeding Prepare 15 plates of 100 mm of diameter coated with laminin for 1h (2’4 microg/ml diluted in Borate Buffer) –> in…
OBJECTIVE: To test the expression of PLEXIN-D1 in NON-TRANSFECTED NEUROBLASTOMA SH-SY5Y CELLS 18.12.10 COATING: Prepare 1 plates of 60mm of diameter with 14 coverslips of 12mm. Treatment…
2018.07.02: Coating and seeding Prepare 4 plates of 60 mm of diameter with 14 coverslips (12 mm) Treatment with laminin 1h (2’4 microg/ml diluted in…
2018.06.19: Coating and seeding Prepare 4 plates of 60 mm of diameter with 14 coverslips (12 mm) Treatment with laminin 1h (2’4 microg/ml diluted in…
Objective: to see if the cells support the incubation with serum (without fixation) –> to set up the IP. Coating and seeding: 04.06.18 [see the…
2018.06.04: Coating and seeding Prepare 4 plates of 35 mm of diameter with 5 coverslips (12 mm) Treatment with: Poly-L-Lysine 1h (0’1 mg/ml diluted in Borate…
Coating and seeding: 28.05.18 [see the post 20180601: ICC HTB-11 neurons (different cell fixation methods) ] ICC protocol: Take 14 coverslips and put them in…
Coating and seeding: 28.05.18 [see the post 20180601: ICC HTB-11 neurons (different cell fixation methods) ] ICC protocol: 2 different protocols Fixation before the ICC…