2018.05.28: Coating and seeding Prepare 2 plates of 60mm of diameter with 14 coverslips (12 mm) Treatment with Poly-D-Lysine (diluted 1/40 with PBS) Incubate for…
Coating and seeding: 22.05.18 [see the post 20180522 – 20180529: HTB-11 cells diferentiation (exp. 4) + ICC] ICC protocol: Fix with PFA 4% (aprox 5…
Day 0- 2018.05.22: Coating and seeding Prepare 2 plates of 60mm of diameter with 14 coverslips (12 mm) Treatment with Poly-D-Lysine (diluted 1/40 with PBS)…
Day 0- 2018.05.14: Coating and seeding Prepare plates of 60mm of diameter with 14 coverslips (12 mm) Treatment with Poly-D-Lysine (diluted 1/40 with PBS) Incubate…
HTB-11 ICC: Samples: Patient’s sera diluted 1/100 with 5 % Goat Serum. Secondary antibody: GAH488 diluted 1/1000 Vectashield mounting medium Results: 102 + The…
Diferenciació HTB-11 a neurones RA 10 µM Medi amb 10 % FBS RA 5 µM + Chol 5 µg/ml Medi amb 10 % FBS NGF…
Diferenciació HTB-11 a neurones Dia 0 –> Sembrar cèl·lules Sembrar a placa de 24 pous 5000 cèls/cm2 (coating amb gelatina 0’15 %) Cada pou té 1’82…
HTB-11 ICC: Samples: Patient’s sera diluted 1/100 with 5 % Goat Serum. Secondary antibody: GAH488 diluted 1/1000 Vectashield mounting medium Results: 18-557 + 18-560…
HTB-11 ICC: Samples: Patient’s sera diluted 1/100 with 5 % Goat Serum. Secondary antibody: GAH488 diluted 1/1000 Vectashield mounting medium Results: 18-539 + 18-543 ++…
HTB-11 ICC: Samples: Patient’s sera diluted 1/100 with 5 % Goat Serum. Secondary antibody: GAH488 diluted 1/1000 Vectashield mounting medium Results: El paciente 18-517 es…