Objective: to test IgG reactivity against SH-SY5Y neuroblastoma derived neurons in patients iwht small fiber neuropathy ICC protocol: Fix diferenciated SH-SY5Y cells with 4% PFA…
Samples: Serum/antibody used Secondary Ab Result 15-198 (CNTN1+ patient) 1/100 GAH 488 IgG + + + CIDP 44 (CNTN1+ patient) 1/100 GAH 488 IgG +…
Objective: try to detect CNTN1 in neuroblastoma cells (indiferentiated) using a higher amount of protein. Try a precast gel 4-15%. Samples/wells: Marker HTB11 cells (23.04.19):…
Objective: try to detect CNTN1 in neuroblastoma neurons (HTB11 differentiated), and in swine sciatic nerve lysate. Samples/wells: Marker Recombinant protein CNTN1: 15 ul + 15…
2019.03.28:seed cells (in proliferation medium) Coating with laminin 2’5 µg/ml diluted in Borate Buffer (for 1 h at 37ºC) Wash 2-3 times with PBS Seed aprox…
2019.03.28:seed cells (in proliferation medium) Coating with laminin 2’5 µg/ml diluted in Borate Buffer (for 1 h at 37ºC) Wash 2-3 times with PBS Seed aprox…
2019.03.28:seed cells (in proliferation medium) Coating with laminin 2’5 µg/ml diluted in Borate Buffer (for 1 h at 37ºC) Wash 2-3 times with PBS Seed aprox…
Objective: try to detect CNTN1 in neuroblastoma neurons (HTB11 differentiated), and in swine sciatic nerve lysate. Samples/wells: Marker Recombinant protein CNTN1: 15 ul + 15…
Objective: We use the product of the IP done on 28.03.2019 (C+, C-), to see if we can detect the CNTN1 in the positive control.…
2019.03.19:seed cells (in proliferation medium) Coating with laminin 2’5 µg/ml diluted in Borate Buffer (for 1 h at 37ºC) Wash 2-3 times with PBS Seed aprox…