Samples:
| Serum/antibody used | Secondary Ab | Result |
| 15-198 (CNTN1+ patient) 1/100 | GAH 488 IgG | + + + |
| CIDP 44 (CNTN1+ patient) 1/100 | GAH 488 IgG | + + + |
| Negative control (serum) 1/100 | GAH 488 IgG | – |
| anti-CASPR2 (rabbit) 1/100 | GAR 488 IgG | – |
| anti-CASPR1 (rabbit) 1/100 | GAR 488 IgG | – |
| anti-CASPR1 (mouse) 1/100 | GAM 488 IgG | – |
| anti-NF (chicken) 1/100 | GAC 488 IgG | + + + |
Protocol:
- Cells seeded on 27/06/2019 (see http://s811335031.mialojamiento.es/lab/blog/2019/04/24/protocol-neuroblastoma-cells-differentiation-and-icc/ )
- Fix for 15 minutes with PFA 4 %
- Wash 3 times with PBS1x
- Block for 1 hour with Goat Serum 5 %
- Patient’s sera
- For IgG: diluted 1/100 in Goat serum 5 % (1 h)
- Wash 3 times with PBS1x
- Secondary antibodies 1/500 1h
- Wash 3 – 6 times with PBS1x
- Vectashield mounting medium
