Samples:
| Sample Id | Result (IgG) | Sample Id | Result (IgG) |
| CIDP 23 | – | CIDP 30 | – |
| CIDP 24 | – | CIDP 31 | + |
| CIDP 25 | – | CIDP 32 | – |
| CIDP 26 | – | Pos. control 15-198 | + + + |
| CIDP 27 | – | Neg control | – |
| CIDP 28 | – | ||
| CIDP 29 | – |
Protocol:
- Cells seeded on 27.06.19 (fixed and blocked on 03.07.19)
- Fix for 15 minutes with PFA 4 %
- Wash 3 times with PBS1x
- Block for 1 hour with Goat Serum 5 %
- Patient’s sera
- For IgG: diluted 1/100 in Goat serum 5 % (1 h)
- Wash 3 times with PBS1x
- Commercial antibody: anti-NF (AF3235) diluted 1/200 in Goat serum 5% (1h)
- Wash 3 times with PBS1x
- Secondary antibodies: GAC594 IgG + GAH488 IgG diluted 1/500 in Goat serum 5 % (1 h)
- Wash 3 – 6 times with PBS1x (NO H2Od)
- Vectashield mounting medium
