Objective: try to detect CNTN1 in neuroblastoma cells (indiferentiated) using a higher amount of protein. Try a precast gel 4-15%. Samples/wells: Marker HTB11 cells (23.04.19):…
Objective: To isolate DNA from leucocyte pellet using the Qiamp DNA Blood Mini kit (51104 Qiagen) Sample: leucocite pellet –> Patient from Klinisches institut für Neurologie (Wien):…
Objectives: try to detect CNTN1 in a mouse brain lysate Try 3 different lysis buffers: nicholson, RIPA+laemmi, lysis buffer IP+Lane sample marker Try 2 different…
Objective: try to detect CNTN1 in neuroblastoma neurons (HTB11 differentiated), and in swine sciatic nerve lysate. Samples/wells: Marker Recombinant protein CNTN1: 15 ul + 15…
ICC NF155: Blocking solution: Goat serum 5 % diluted in PBS. Patient’s sera diluted 1/100 Primary antibody (anti-NF AF3235) diluted 1/1000. Secondary antibodies: GAC 488…
Objective: try to detect CNTN1 in neuroblastoma neurons (HTB11 differentiated), and in swine sciatic nerve lysate. Samples/wells: Marker Recombinant protein CNTN1: 15 ul + 15…
Samples: For IgG – 89-116, Positive control, negative control. For IgM: 89-112, negative control. CNTN1 ICC: Blocking solution: Rabbit serum diluted 1/40 in PBS. Patient’s…
Objective: To test IgG reactivity towards CNTN1 in GBS samples from the spanish cohort of IGOS. We are going to try again with 5% milk…
We want to set up the detection of CNTN1 by WB (with Odyssey technology), and also we want to test the detection of CNTN1 in…
Objectives: To test CMT patients for IgM positivity towards CNTN1. Samples: 58-74, negative control (11). CNTN1 ICC: Blocking solution: Rabbit serum diluted 1/40 in PBS.…