Objectives: To test CMT patients for IgM positivity towards CNTN1.
Samples: 58-74, negative control (11).
CNTN1 ICC:
- Blocking solution: Rabbit serum diluted 1/40 in PBS.
- Patient’s sera diluted 1/40 with rabbit serum diluted 1/40 in PBS.
- Primary antibody: AF904 diluted 1/1000.
- Secondary antibodies: RAG 488 + RAH 594 IgM (1/1000).
- Vectashield mounting medium.
RESULTS:
All samples were negative. Sample 64 had no cells. We are going to repeat the ICC in sample 64.
