Objective: To test CMT patients for IgG positivity towards NF155.
Samples: 64-85, negative control (11), positive control (LAC).
ICC NF155:
- Blocking solution: 5% goat serum in PBS.
- Patient’s sera diluted 1/100 for IgG with 5% goat serum in PBS.
- Primary antibody: AF3235 diluted 1/1000.
- Secondary antibodies: GAC488 + GAH 594 IgM (1/1000).
- Vectashield mounting medium
RESULTS: All samples were negative.
