Samples: For IgG – 89-116, Positive control, negative control. For IgM: 89-112, negative control.
CNTN1 ICC:
- Blocking solution: Rabbit serum diluted 1/40 in PBS.
- Patient’s sera diluted 1/100 for IgG with rabbit serum 1/40.
- Primary antibody: AF904 diluted 1/1000.
- Secondary antibodies: RAG 488 + RAH 594 IgM (1/1000)
- Vectashield mounting medium.
RESULTS: All samples were negative.
