HTB-11 ICC: Samples: Patient’s sera diluted 1/100 with 5 % Goat Serum. Secondary antibody: GAH488 diluted 1/1000 Vectashield mounting medium Results: El paciente 18-517 es…
Day 1- 2018.04.16: Trypsin-EDTA a plate of HEK293, count cells, and prepare 3 prepare 3 plates of 100mm of diameter (one plate for non-transfected HEK293…
2018.04.16: Prepare 4 plates of 60mm of diameter with 24 coverslips (9 mm) Treatment with Poly-D-Lysine (diluted 1/40 with PBS) Incubate for 1h (37ºC, 5%…
Day 1- 2018.04.16: Prepare 4 plates of 60mm of diameter with 24 coverslips (9 mm) Treatment with Poly-D-Lysine (diluted 1/40 with PBS) Incubate for 1h…
DRG NEURONS ICC Incubate with patient’s sera in a 24 wells plate: 1 hour 37ºC (prepare the dilution in the wells and then put one…
2018.04.09: Prepare 1 plate of 60mm of diameter with 24 coverslips (9 mm) Treatment with Poly-D-Lysine (diluted 1/40 with boratte buffer) Incubate for 1h (37ºC,…
Objectives: To test CMT patients for IgG positivity towards NF155. Samples: NF155 ICC: HEKs 293 Cells Transfection: 2018.01.16 Blocking solution: 5% goat serum in PBS.…
Day 1- 2018.04.09: Prepare 3 plates of 60mm of diameter with 24 coverslips (9 mm) Treatment with Poly-D-Lysine (diluted 1/40 with boratte buffer) Incubate for…
Objectives: To test Multiple Sclerosis patients muscle for IgG positivity towards ANO2 Patients: 14-15402 (Miguel Ángel Ordoño Moreno –> Multiple Esclerosis) 15-4507 (control) Immunohistochemistry…
Objectives: To test Multiple Sclerosis patients for IgG positivity towards ANO2 Patients: 212 to 237 (from the Multiple Sclerosis sera collection) ANO2 ICC: HEKs 293…