Objectives: To test CMT patients for IgG positivity towards NF155.
Samples:
| NAME (Diagnosis) | CODE | RESULTS IgG |
|---|---|---|
| Gerardo Martínez Martínez (CMT) | 213-18 | - |
| Neus Assens Serra (CMT1A) | 18-622 | - |
| Roger Marginet Assens (CMT1A) | 18-623 | - |
| Neuromus_184 (H. La Fe) (CMT) | 18-624 | - |
| Neuromus_185 (H. La Fe) (CMT) | 18-625 | - |
| Neuromus_194 (H. La Fe) (CMT) | 18-626 | - |
| Neuromus_195 (H. La Fe) (CMT) | 18-627 | - |
| Neuromus_196 (H. La Fe) (CMT) | 18-628 | - |
| Neuromus_202 (H. La Fe) (CMT) | 18-629 | - |
| Neuromus_203 (H. La Fe) (CMT) | 18-630 | - |
| Neuromus_204 (H. La Fe) (CMT) | 18-631 | - |
| Neuromus_218 (H. La Fe) (CMT) | 18-632 | - |
| Neuromus_219 (H. La Fe) (CMT) | 18-633 | - |
| Neuromus_258 (H. La Fe) (CMT) | 18-634 | - |
| Neuromus_316 (H. La Fe) (CMT) | 18-635 | - |
| Control negatiu | - | |
| Control positiu | 15-195 | + |
NF155 ICC:
- HEKs 293 Cells Transfection: 2018.01.16
- Blocking solution: 5% goat serum in PBS.
- Patient’s sera diluted 1/100 for IgG with 5% goat serum in PBS.
- Primary antibody: AF3235 diluted 1/1000.
- Secondary antibodies: GAC 488 (1/1000) + GAH 594 IgG (1/1000)
- Vectashield mounting medium.
Results: all samples were negative for IgG towards NF155.
