2021.12.27 – 2022.02.03: ELISA Gangliósidos (screening EM vacunas)

Objetivo: testar 100 muestras del estudio “Efficacy and neurological adverse effects of COVID-19 vaccination in patients immunocompromised for multiple sclerosis“. Todo este screening inicial lo hizo Julia (estudiante de técnico de laboratorio)

Protocolo general:

Preparación placas ELISA gangliósidos

  • Reconstituir los viales de gangliósidos con 1 ml de cloroformo/metanol (1:1), excepto en el caso de GQ1b que se reconstituyen con 0,5ml. Una vez reconstituidos, se guardan a -20ºC
    • GM1: 1 mg + 1 ml: 1 mg/ml
    • GD1b: 1 mg  + 1 ml: 1 mg/ml
    • GQ1b: 0,5 mg + 0,5 ml: 1 mg/ml
  • Diseñar placas: en este caso se harán así:
 123456789101112
AblancoGM1GD1bGQ1bblancoGM1GD1bGQ1bblancoGM1GD1bGQ1b
BblancoGM1GD1bGQ1bblancoGM1GD1bGQ1bblancoGM1GD1bGQ1b
CblancoGM1GD1bGQ1bblancoGM1GD1bGQ1bblancoGM1GD1bGQ1b
DblancoGM1GD1bGQ1bblancoGM1GD1bGQ1bblancoGM1GD1bGQ1b
EblancoGM1GD1bGQ1bblancoGM1GD1bGQ1bblancoGM1GD1bGQ1b
FblancoGM1GD1bGQ1bblancoGM1GD1bGQ1bblancoGM1GD1bGQ1b
GblancoGM1GD1bGQ1bblancoGM1GD1bGQ1bblancoGM1GD1bGQ1b
HblancoGM1GD1bGQ1bblancoGM1GD1bGQ1bblancoGM1GD1bGQ1b
  • Preparar la solución de metanol+gangliósido en función de las placas que se vayan a preparar. La concentración final es de 2 ug/ml (excepto GQ1b que es 4 ug/ml).

Para 5 placas (3 columnas de cada gangliósido/placa):

  • GM1: 15 ml metanol + 30 ul GM1 reconstituído
    • GD1b: 15 ml metanol + 30 ul GD1b reconstituído
    • GQ1b: 15 ml metanol + 30 ul GQ1b reconstituído
  • Agitar con vórtex las diluciones
  • Poner 100 ul de gangliósido diluído a cada pozo (en la columna que corresponda). En los pozos del blanco se pone sólo metanol
  • Dejar secar las placas a temperatura ambiente (preferiblemente en campana extractora) hasta que se evapore el metanol (mínimo 4 horas)
  • Congelar las placas a -20ºC

ELISA Gangliósidos día 1

Todas las muestras se hacen por IgG y por IgM, a dilución 1/100 y 1/500 (en total se usarán 16 pozos para cada muestra). En cada placa podremos testar 5 muestras y los controles.

  • Descongelar placas con gangliósidos
  • Bloquear con PBS-BSA 1 % : 200 ul/pozo, incubar 2h a 4ºC
  • Lavar 2 veces con PBS (en cubeta)
  • Preparar diluciones de sueros (en PBS-BSA 0’1%)
    • Dil 1/100 (x8 pozos): 1 ml PBS-BSA 0’1% + 10 ul suero
    • Dil 1/500 (x8 pozos): 0,8 ml de PBS-BSA 0’1% + 0,2 ml de la dilución anterior.
      • En el caso de los controles, como ponemos un suero para IgG y otro para IgM, preparamos la mitad de cada dilución
  • Poner 100 ul de suero a cada pozo, incubar toda la noche a 4ºC

*Ejemplo: si testamos las muestras A1, A2, A3, A4, A5 el esquema de la placa sería el siguiente:

 123456789101112
A IgGA1 1/100A1 1/100A1 1/100A1 1/100A2 1/100A2 1/100A2 1/100A2 1/100A3 1/100A3 1/100A3 1/100A3 1/100
B IgGA1 1/500A1 1/500A1 1/500A1 1/500A2 1/500A2 1/500A2 1/500A2 1/500A3 1/500A3 1/500A3 1/500A3 1/500
C IgGA4 1/100A4 1/100A4 1/100A4 1/100A5 1/100A5 1/100A5 1/100A5 1/100C+ IgG 1/100C+ IgG 1/100C+ IgG 1/100C+ IgG 1/100
D IgGA4 1/500A4 1/500A4 1/500A4 1/500A5 1/500A5 1/500A5 1/500A5 1/500C+ IgG 1/500C+ IgG 1/500C+ IgG 1/500C+ IgG 1/500
E IgMA1 1/100A1 1/100A1 1/100A1 1/100A2 1/100A2 1/100A2 1/100A2 1/100A3 1/100A3 1/100A3 1/100A3 1/100
F IgMA1 1/500A1 1/500A1 1/500A1 1/500A2 1/500A2 1/500A2 1/500A2 1/500A3 1/500A3 1/500A3 1/500A3 1/500
G IgMA4 1/100A4 1/100A4 1/100A4 1/100A5 1/100A5 1/100A5 1/100A5 1/100C+IgM 1/100C+ IgM 1/100C+ IgM 1/100C+ IgM 1/100
H IgMA4 1/500A4 1/500A4 1/500A4 1/500A5 1/500A5 1/500A5 1/500A5 1/500C+ IgM 1/500C+ IgM 1/500C+ IgM 1/500C+ IgM 1/500

ELISA Gangliósidos día 2

  • Lavar 4 veces con PBS
  • Preparar Ac secundarios: RAH HRP IgG o IgM dil. 1/5000 en PBS-BSA 0’1%. Poner 100 ul de la dilución a cada pozo, incubar 2h a 4ºC
  • Lavar 4 veces con PBS
  • Preparar  el sustrato: 1 tableta de OPD + 1 tableta de urea hydrogen peroxidase + 20 ml agua destilada (proteger de la luz con papel de aluminio y agitar). Poner 100 ul a cada pozo, incubar 40 min a temperatura ambiente
  • Parar la reacción añadiendo 50 ul de H2SO4 al 25% a cada pozo.
  • Leer en lector de placas de ELISA a 490-630 nm

Resultados:

28/12/21

 1 blanc2 gm13 gd1b4 gq1b5 blanc6 gm17 gd1b8 gq1b9 blanc10 gm111 gd1b12 gq1b
A4206179 1/1004206179 1/1004206179 1/1004206179 1/100575657 1/100575657 1/100575657 1/100575657 1/10027419 1/10027419 1/10027419 1/10027419 1/100
B4206179 1/5004206179 1/5004206179 1/5004206179 1/500575657 1/500575657 1/500575657 1/500575657 1/50027419 1/50027419 1/50027419 1/50027419 1/500
C70198581 1/10070198581 1/10070198581 1/10070198581 1/10070525486 1/10070525486 1/10070525486 1/10070525486 1/100C+ IgG 1/100C+ IgG 1/100C+ IgG 1/100C+ IgG 1/100
D70198581 1/50070198581 1/50070198581 1/50070198581 1/50070525486 1/50070525486 1/50070525486 1/50070525486 1/500C+ IgG 1/500C+ IgG 1/500C+ IgG 1/500C+ IgG 1/500
E4206179 1/1004206179 1/1004206179 1/1004206179 1/100575657 1/100575657 1/100575657 1/100575657 1/10027419 1/10027419 1/10027419 1/10027419 1/100
F4206179 1/5004206179 1/5004206179 1/5004206179 1/500575657 1/500575657 1/500575657 1/500575657 1/50027419 1/50027419 1/50027419 1/50027419 1/500
G70198581 1/10070198581 1/10070198581 1/10070198581 1/10070525486 1/10070525486 1/10070525486 1/10070525486 1/100C+ IgM 1/100C+ IgM 1/100C+ IgM 1/100C+ IgM 1/100
H70198581 1/50070198581 1/50070198581 1/50070198581 1/50070525486 1/50070525486 1/50070525486 1/50070525486 1/500C+ IgM 1/500C+ IgM 1/500C+ IgM 1/500C+ IgM 1/500
 123456789101112
A2,8571,5052,6532,3520,8590,9421,1730,8211,0861,150,8410,937
B1,4091,1331,3460,9660,4980,4460,6480,6690,5480,8290,5280,428
C1,4831,531,8491,2131,9211,7282,5891,7021,8273,6393,6043,432
D0,7170,8680,830,7991,2380,8651,1681,2071,3443,5993,4932,9
E2,4812,662,9932,7851,4140,5960,9230,6791,9590,7121,3130,802
F1,8451,4441,7651,4380,5630,2830,4560,2871,1950,4290,6070,338
G1,3061,3860,8960,6661,2591,1051,1410,8311,9841,7642,9392,26
H0,6890,6120,3740,2780,6050,4770,4240,3251,2551,1482,7311,772

29/12/21

 1 blanc2 gm13 gd1b4 gq1b5 blanc6 gm17 gd1b8 gq1b9 blanc10 gm111 gd1b12 gq1b
A4000789 1/1004000789 1/1004000789 1/1004000789 1/100607739 1/100607739 1/100607739 1/100607739 1/1005189386 1/1005189386 1/1005189386 1/1005189386 1/100
B4000789 1/5004000789 1/5004000789 1/5004000789 1/500607739 1/500607739 1/500607739 1/500607739 1/5005189386 1/5005189386 1/5005189386 1/5005189386 1/500
C4688595 1/1004688595 1/1004688595 1/1004688595 1/1004331847 1/1004331847 1/1004331847 1/1004331847 1/100C+ IgG 1/100C+ IgG 1/100C+ IgG 1/100C+ IgG 1/100
D4688595 1/5004688595 1/5004688595 1/5004688595 1/5004331847 1/5004331847 1/5004331847 1/5004331847 1/500C+ IgG 1/500C+ IgG 1/500C+ IgG 1/500C+ IgG 1/500
E4000789 1/1004000789 1/1004000789 1/1004000789 1/100607739 1/100607739 1/100607739 1/100607739 1/1005189386 1/1005189386 1/1005189386 1/1005189386 1/100
F4000789 1/5004000789 1/5004000789 1/5004000789 1/500607739 1/500607739 1/500607739 1/500607739 1/5005189386 1/5005189386 1/5005189386 1/5005189386 1/500
G4688595 1/1004688595 1/1004688595 1/1004688595 1/1004331847 1/1004331847 1/1004331847 1/1004331847 1/100C+ IgM 1/100C+ IgM 1/100C+ IgM 1/100C+ IgM 1/100
H4688595 1/5004688595 1/5004688595 1/5004688595 1/5004331847 1/5004331847 1/5004331847 1/5004331847 1/500C+ IgM 1/500C+ IgM 1/500C+ IgM 1/500C+ IgM 1/500
 123456789101112
A1,7511,1091,151,251,3341,0860,9191,5221,761,4061,4981,234
B0,60,560,5480,5140,8220,7680,6340,60,7970,8480,7910,585
C0,6420,6410,5010,4751,1850,9430,8570,831,5783,6873,6863,535
D0,2930,3230,2960,3230,4890,5620,4030,4980,9943,663,6141,806
E1,1880,7870,6360,5421,5881,281,2711,2091,5621,520,9920,854
F0,6190,3820,2860,2741,0070,7580,580,5580,9160,6490,420,392
G0,4590,3730,3060,2690,60,3260,2810,2281,5961,6462,9032,244
H0,2050,1770,1570,1270,3480,1850,170,1670,9320,9972,6831,218

30/12/21

 1 blanc2 gm13 gd1b4 gq1b5 blanc6 gm17 gd1b8 gq1b9 blanc10 gm111 gd1b12 gq1b
A139910 1/100139910 1/100139910 1/100139910 1/1004213704 1/1004213704 1/1004213704 1/1004213704 1/10070598300 1/10070598300 1/10070598300 1/10070598300 1/100
B139910 1/500139910 1/500139910 1/500139910 1/5004213704 1/5004213704 1/5004213704 1/5004213704 1/50070598300 1/50070598300 1/50070598300 1/50070598300 1/500
C391156 1/100391156 1/100391156 1/100391156 1/1004065729 1/1004065729 1/1004065729 1/1004065729 1/100C+ IgG 1/100C+ IgG 1/100C+ IgG 1/100C+ IgG 1/100
D391156 1/500391156 1/500391156 1/500391156 1/5004065729 1/5004065729 1/5004065729 1/5004065729 1/500C+ IgG 1/500C+ IgG 1/500C+ IgG 1/500C+ IgG 1/500
E139910 1/100139910 1/100139910 1/100139910 1/1004213704 1/1004213704 1/1004213704 1/1004213704 1/10070598300 1/10070598300 1/10070598300 1/10070598300 1/100
F139910 1/500139910 1/500139910 1/500139910 1/5004213704 1/5004213704 1/5004213704 1/5004213704 1/50070598300 1/50070598300 1/50070598300 1/50070598300 1/500
G391156 1/100391156 1/100391156 1/100391156 1/1004065729 1/1004065729 1/1004065729 1/1004065729 1/100C+ IgM 1/100C+ IgM 1/100C+ IgM 1/100C+ IgM 1/100
H391156 1/500391156 1/500391156 1/500391156 1/5004065729 1/5004065729 1/5004065729 1/5004065729 1/500C+ IgM 1/500C+ IgM 1/500C+ IgM 1/500C+ IgM 1/500
 123456789101112
A0,9412,1951,1760,7611,4810,9531,2130,8521,5211,9181,4551,483
B0,6070,8390,4470,5790,8810,5340,6190,3420,9420,8150,5970,522
C1,1091,3021,120,8751,2211,5370,9531,1872,2543,7073,6933,629
D0,5140,5590,7840,6810,7890,9280,7470,7711,5023,7023,6532,985
E0,6690,3810,4310,31,1120,4490,4160,3121,1411,110,8520,6
F0,2990,170,1870,1460,3920,1910,2040,1540,5170,4320,2890,251
G0,7860,380,2890,2821,4631,2191,0510,7611,5771,8692,8521,886
H0,3850,1860,1460,150,8370,5010,3940,3050,9120,9772,9051,232

04/01/22

 1 blanc2 gm13 gd1b4 gq1b5 blanc6 gm17 gd1b8 gq1b9 blanc10 gm111 gd1b12 gq1b
A70600773 1/10070600773 1/10070600773 1/10070600773 1/1004651567 1/1004651567 1/1004651567 1/1004651567 1/1004527218 1/1004527218 1/1004527218 1/1004527218 1/100
B70600773 1/50070600773 1/50070600773 1/50070600773 1/5004651567 1/5004651567 1/5004651567 1/5004651567 1/5004527218 1/5004527218 1/5004527218 1/5004527218 1/500
C4225069 1/1004225069 1/1004225069 1/1004225069 1/1005188010 1/1005188010 1/1005188010 1/1005188010 1/100C+ IgG 1/100C+ IgG 1/100C+ IgG 1/100C+ IgG 1/100
D4225069 1/5004225069 1/5004225069 1/5004225069 1/5005188010 1/5005188010 1/5005188010 1/5005188010 1/500C+ IgG 1/500C+ IgG 1/500C+ IgG 1/500C+ IgG 1/500
E70600773 1/10070600773 1/10070600773 1/10070600773 1/1004651567 1/1004651567 1/1004651567 1/1004651567 1/1004527218 1/1004527218 1/1004527218 1/1004527218 1/100
F70600773 1/50070600773 1/50070600773 1/50070600773 1/5004651567 1/5004651567 1/5004651567 1/5004651567 1/5004527218 1/5004527218 1/5004527218 1/5004527218 1/500
G4225069 1/1004225069 1/1004225069 1/1004225069 1/1005188010 1/1005188010 1/1005188010 1/1005188010 1/100C+ IgM 1/100C+ IgM 1/100C+ IgM 1/100C+ IgM 1/100
H4225069 1/5004225069 1/5004225069 1/5004225069 1/5005188010 1/5005188010 1/5005188010 1/5005188010 1/500C+ IgM 1/500C+ IgM 1/500C+ IgM 1/500C+ IgM 1/500
 123456789101112
A0,5270,6610,6240,4920,6940,8710,9430,9250,8180,8710,590,54
B0,3060,4870,3620,4340,3910,5270,5060,4540,4350,5060,5040,583
C0,921,3690,7720,9370,5990,560,4630,61,5253,683,683,552
D0,4110,5140,3750,3840,2450,4680,2860,2340,8053,6323,5812,423
E1,20,7150,6630,6840,8720,6810,6070,4060,5930,3640,260,246
F0,5360,2990,3190,2260,4480,3190,2790,2120,3780,180,1770,152
G0,60,280,2920,240,3070,2320,1840,1811,1781,2972,6941,764
H0,3130,1560,1550,1210,1540,1230,1060,1050,6160,7491,9550,92

05/01/22

 1 blanc2 gm13 gd1b4 gq1b5 blanc6 gm17 gd1b8 gq1b9 blanc10 gm111 gd1b12 gq1b
A530911 1/100530911 1/100530911 1/100530911 1/10070725393 1/10070725393 1/10070725393 1/10070725393 1/1004166275 1/1004166275 1/1004166275 1/1004166275 1/100
B530911 1/500530911 1/500530911 1/500530911 1/50070725393 1/50070725393 1/50070725393 1/50070725393 1/5004166275 1/5004166275 1/5004166275 1/5004166275 1/500
C5212994 1/1005212994 1/1005212994 1/1005212994 1/100364771 1/100364771 1/100364771 1/100364771 1/100C+ IgG 1/100C+ IgG 1/100C+ IgG 1/100C+ IgG 1/100
D5212994 1/5005212994 1/5005212994 1/5005212994 1/500364771 1/500364771 1/500364771 1/500364771 1/500C+ IgG 1/500C+ IgG 1/500C+ IgG 1/500C+ IgG 1/500
E70600773 1/10070600773 1/10070600773 1/10070600773 1/1004651567 1/1004651567 1/1004651567 1/1004651567 1/1004527218 1/1004527218 1/1004527218 1/1004527218 1/100
F70600773 1/50070600773 1/50070600773 1/50070600773 1/5004651567 1/5004651567 1/5004651567 1/5004651567 1/5004527218 1/5004527218 1/5004527218 1/5004527218 1/500
G4225069 1/1004225069 1/1004225069 1/1004225069 1/1005188010 1/1005188010 1/1005188010 1/1005188010 1/100C+ IgM 1/100C+ IgM 1/100C+ IgM 1/100C+ IgM 1/100
H4225069 1/5004225069 1/5004225069 1/5004225069 1/5005188010 1/5005188010 1/5005188010 1/5005188010 1/500C+ IgM 1/500C+ IgM 1/500C+ IgM 1/500C+ IgM 1/500
 123456789101112
A0,6840,9050,510,4990,3980,3160,340,2790,8120,630,6470,397
B0,330,3250,2390,2610,2280,2280,1930,2380,3730,3090,3580,212
C0,9621,080,6670,840,6840,9150,7310,4681,1083,6273,6333,405
D0,1520,1690,1570,1440,3630,5140,3240,3360,9783,6193,551,493
E0,2390,2410,1850,180,6040,5780,6780,5011,1960,6170,5220,302
F0,1660,1160,0940,1220,2050,2420,2660,2320,5280,260,2250,155
G0,210,1940,1350,1440,5260,3630,350,2750,9450,9611,9791,041
H0,0820,1130,0770,0750,2440,160,150,1250,5450,5961,3850,569

11/01/2022

 1 blanc2 gm13 gd1b4 gq1b5 blanc6 gm17 gd1b8 gq1b9 blanc10 gm111 gd1b12 gq1b
A724222 1/100724222 1/100724222 1/100724222 1/100535889 1/100535889 1/100535889 1/100535889 1/1004858806 1/1004858806 1/1004858806 1/1004858806 1/100
B724222 1/500724222 1/500724222 1/500724222 1/500535889 1/500535889 1/500535889 1/500535889 1/5004858806 1/5004858806 1/5004858806 1/5004858806 1/500
C5211331 1/1005211331 1/1005211331 1/1005211331 1/10080075933 1/10080075933 1/10080075933 1/10080075933 1/100C+ IgG 1/100C+ IgG 1/100C+ IgG 1/100C+ IgG 1/100
D5211331 1/5005211331 1/5005211331 1/5005211331 1/50080075933 1/50080075933 1/50080075933 1/50080075933 1/500C+ IgG 1/500C+ IgG 1/500C+ IgG 1/500C+ IgG 1/500
E724222 1/100724222 1/100724222 1/100724222 1/100535889 1/100535889 1/100535889 1/100535889 1/1004858806 1/1004858806 1/1004858806 1/1004858806 1/100
F724222 1/500724222 1/500724222 1/500724222 1/500535889 1/500535889 1/500535889 1/500535889 1/5004858806 1/5004858806 1/5004858806 1/5004858806 1/500
G5211331 1/1005211331 1/1005211331 1/1005211331 1/10080075933 1/10080075933 1/10080075933 1/10080075933 1/100C+ IgM 1/100C+ IgM 1/100C+ IgM 1/100C+ IgM 1/100
H5211331 1/5005211331 1/5005211331 1/5005211331 1/50080075933 1/50080075933 1/50080075933 1/50080075933 1/500C+ IgM 1/500C+ IgM 1/500C+ IgM 1/500C+ IgM 1/500
 123456789101112
A0,5210,3120,2230,2140,7640,5770,390,3190,3210,330,2460,249
B0,2460,1660,1450,1770,3390,2510,2020,1560,2010,1730,1260,112
C0,4010,370,3210,3760,8040,4340,3770,3221,0143,6113,6193,378
D0,2430,2050,1620,1770,3940,2530,1750,1550,4513,543,4531,961
E1,1450,7560,6530,5930,6650,480,3410,0780,4630,30,2930,197
F0,7080,3780,2720,220,3080,1940,1520,1330,2150,140,1260,107
G0,7710,4720,4180,2531,7531,1281,0370,991,7611,4422,9242,377
H0,4540,2260,2240,1570,8080,4240,4220,4060,8580,6032,5521,384

13/01/2022

 1 blanc2 gm13 gd1b4 gq1b5 blanc6 gm17 gd1b8 gq1b9 blanc10 gm111 gd1b12 gq1b
A4870305 1/1004870305 1/1004870305 1/1004870305 1/100683633 1/100683633 1/100683633 1/100683633 1/100368893 1/100368893 1/100368893 1/100368893 1/100
B4870305 1/5004870305 1/5004870305 1/5004870305 1/500683633 1/500683633 1/500683633 1/500683633 1/500368893 1/500368893 1/500368893 1/500368893 1/500
C70177507 1/10070177507 1/10070177507 1/10070177507 1/1004387159 1/1004387159 1/1004387159 1/1004387159 1/100C+ IgM 1/100C+ IgM 1/100C+ IgM 1/100C+ IgM 1/100
D70177507 1/50070177507 1/50070177507 1/50070177507 1/5004387159 1/5004387159 1/5004387159 1/5004387159 1/500C+ IgM 1/500C+ IgM 1/500C+ IgM 1/500C+ IgM 1/500
E4870305 1/1004870305 1/1004870305 1/1004870305 1/100683633 1/100683633 1/100683633 1/100683633 1/100368893 1/100368893 1/100368893 1/100368893 1/100
F4870305 1/5004870305 1/5004870305 1/5004870305 1/500683633 1/500683633 1/500683633 1/500683633 1/500368893 1/500368893 1/500368893 1/500368893 1/500
G70177507 1/10070177507 1/10070177507 1/10070177507 1/1004387159 1/1004387159 1/1004387159 1/1004387159 1/100C+ IgG 1/100C+ IgG 1/100C+ IgG 1/100C+ IgG 1/100
H70177507 1/50070177507 1/50070177507 1/50070177507 1/5004387159 1/5004387159 1/5004387159 1/5004387159 1/500C+ IgG 1/500C+ IgG 1/500C+ IgG 1/500C+ IgG 1/500

*Va posar els Ac secundaris al revés

 123456789101112
A1,7110,6250,6150,4971,0490,4510,4150,3560,8230,8480,6780,562
B1,1850,3270,2870,2420,5230,2580,2230,1880,4660,4160,4470,257
C0,5540,6650,5280,2280,6510,2980,3510,2761,0460,8092,1651,591
D0,230,260,1980,1260,2880,1580,1630,1490,6260,5392,0030,927
E0,5630,4660,4630,711,1430,840,9440,8780,5920,6750,8460,922
F0,3670,2430,2660,3390,5650,4490,4790,5210,3840,3610,5190,552
G0,5940,6560,7110,9710,5620,4590,7510,721,573,9253,9853,342
H0,2560,3050,3870,4790,2880,1950,2580,3130,7153,4013,3621,622

18/01/2022

 1 blanc2 gm13 gd1b4 gq1b5 blanc6 gm17 gd1b8 gq1b9 blanc10 gm111 gd1b12 gq1b
A510557 1/100510557 1/100510557 1/100510557 1/1004952509 1/1004952509 1/1004952509 1/1004952509 1/1004183342 1/1004183342 1/1004183342 1/1004183342 1/100
B510557 1/500510557 1/500510557 1/500510557 1/5004952509 1/5004952509 1/5004952509 1/5004952509 1/5004183342 1/5004183342 1/5004183342 1/5004183342 1/500
C4274088 1/1004274088 1/1004274088 1/1004274088 1/1004964212 1/1004964212 1/1004964212 1/1004964212 1/100C+ IgG 1/100C+ IgG 1/100C+ IgG 1/100C+ IgG 1/100
D4274088 1/5004274088 1/5004274088 1/5004274088 1/5004964212 1/5004964212 1/5004964212 1/5004964212 1/500C+ IgG 1/500C+ IgG 1/500C+ IgG 1/500C+ IgG 1/500
E510557 1/100510557 1/100510557 1/100510557 1/1004952509 1/1004952509 1/1004952509 1/1004952509 1/1004183342 1/1004183342 1/1004183342 1/1004183342 1/100
F510557 1/500510557 1/500510557 1/500510557 1/5004952509 1/5004952509 1/5004952509 1/5004952509 1/5004183342 1/5004183342 1/5004183342 1/5004183342 1/500
G4274088 1/1004274088 1/1004274088 1/1004274088 1/1004964212 1/1004964212 1/1004964212 1/1004964212 1/100C+ IgM  1/100C+ IgM 1/100C+ IgM 1/100C+ IgM 1/100
H4274088 1/5004274088 1/5004274088 1/5004274088 1/5004964212 1/5004964212 1/5004964212 1/5004964212 1/500C+ IgM 1/500C+ IgM 1/500C+ IgM 1/500C+ IgM 1/500
 123456789101112
A1,2831,1291,1641,4661,10,960,8430,881,6671,4911,6571,502
B0,6590,7960,6410,7560,470,4520,370,5791,1840,840,8580,875
C1,041,1590,8220,8980,8830,7650,7820,6541,9043,643,7083,54
D0,4060,4840,4750,370,5310,3920,3760,3481,0653,6143,5872,277
E0,820,7830,5450,3571,0511,0871,3940,6741,2140,7950,7770,613
F0,5010,3180,2590,1730,5140,4450,6410,290,750,4180,3530,317
G0,7190,4490,3620,2921,1430,5290,680,4971,2811,3272,1051,656
H0,2950,210,1760,1920,5540,210,3330,2250,8840,8382,1351,133

19/01/2022

 1 blanc2 gm13 gd1b4 gq1b5 blanc6 gm17 gd1b8 gq1b9 blanc10 gm111 gd1b12 gq1b
A5118199 1/1005118199 1/1005118199 1/1005118199 1/1004920245 1/1004920245 1/1004920245 1/1004920245 1/1004692213 1/1004692213 1/1004692213 1/1004692213 1/100
B5118199 1/5005118199 1/5005118199 1/5005118199 1/5004920245 1/5004920245 1/5004920245 1/5004920245 1/5004692213 1/5004692213 1/5004692213 1/5004692213 1/500
C5175761 1/1005175761 1/1005175761 1/1005175761 1/10070667440 1/10070667440 1/10070667440 1/10070667440 1/100C+ IgG 1/100C+ IgG 1/100C+ IgG 1/100C+ IgG 1/100
D5175761 1/5005175761 1/5005175761 1/5005175761 1/50070667440 1/50070667440 1/50070667440 1/50070667440 1/500C+ IgG 1/500C+ IgG 1/500C+ IgG 1/500C+ IgG 1/500
E510557 1/100510557 1/100510557 1/100510557 1/1004952509 1/1004952509 1/1004952509 1/1004952509 1/1004183342 1/1004183342 1/1004183342 1/1004183342 1/100
F510557 1/500510557 1/500510557 1/500510557 1/5004952509 1/5004952509 1/5004952509 1/5004952509 1/5004183342 1/5004183342 1/5004183342 1/5004183342 1/500
G4274088 1/1004274088 1/1004274088 1/1004274088 1/1004964212 1/1004964212 1/1004964212 1/1004964212 1/100C+ IgM  1/100C+ IgM 1/100C+ IgM 1/100C+ IgM 1/100
H4274088 1/5004274088 1/5004274088 1/5004274088 1/5004964212 1/5004964212 1/5004964212 1/5004964212 1/500C+ IgM 1/500C+ IgM 1/500C+ IgM 1/500C+ IgM 1/500
 123456789101112
A0,6870,6780,6750,7991,471,0231,0341,1061,3361,5611,3451,01
B0,3690,3770,4080,4610,790,6310,670,6330,6850,7690,6750,777
C0,7230,6270,8230,8350,9931,0651,1621,0171,7843,6783,6763,509
D0,4410,3180,5010,3620,5540,5030,4360,4521,0893,6213,6121,497
E0,6550,6520,5530,3391,7140,8090,7150,4531,450,9110,870,681
F0,5920,3030,2390,190,870,3730,2870,2440,8110,4370,390,323
G1,2150,8070,5830,4610,950,5420,5940,4821,2541,3132,6481,729
H0,6430,4390,350,2570,4160,290,3080,2560,9410,7032,5061,3

21/01/2022

 1 blanc2 gm13 gd1b4 gq1b5 blanc6 gm17 gd1b8 gq1b9 blanc10 gm111 gd1b12 gq1b
A641216 1/100641216 1/100641216 1/100641216 1/1005285494 1/1005285494 1/1005285494 1/1005285494 1/1004795584 1/1004795584 1/1004795584 1/1004795584 1/100
B641216 1/500641216 1/500641216 1/500641216 1/5005285494 1/5005285494 1/5005285494 1/5005285494 1/5004795584 1/5004795584 1/5004795584 1/5004795584 1/500
C720643 1/100720643 1/100720643 1/100720643 1/100767614 1/100767614 1/100767614 1/100767614 1/100C+ IgG 1/100C+ IgG 1/100C+ IgG 1/100C+ IgG 1/100
D720643 1/500720643 1/500720643 1/500720643 1/500767614 1/500767614 1/500767614 1/500767614 1/500C+ IgG 1/500C+ IgG 1/500C+ IgG 1/500C+ IgG 1/500
E641216 1/100641216 1/100641216 1/100641216 1/1005285494 1/1005285494 1/1005285494 1/1005285494 1/1004795584 1/1004795584 1/1004795584 1/1004795584 1/100
F641216 1/500641216 1/500641216 1/500641216 1/5005285494 1/5005285494 1/5005285494 1/5005285494 1/5004795584 1/5004795584 1/5004795584 1/5004795584 1/500
G720643 1/100720643 1/100720643 1/100720643 1/100767614 1/100767614 1/100767614 1/100767614 1/100C+ IgM 1/100C+ IgM 1/100C+ IgM 1/100C+ IgM 1/100
H720643 1/500720643 1/500720643 1/500720643 1/500767614 1/500767614 1/500767614 1/500767614 1/500C+ IgM 1/500C+ IgM 1/500C+ IgM 1/500C+ IgM 1/500
 123456789101112
A0,7840,8610,5070,4950,8921,2010,6370,8661,1461,2230,8970,87
B0,4790,490,3190,270,3680,4850,3660,3830,5330,5360,4610,497
C1,1741,3041,0220,8061,2851,3271,1890,8592,2323,6823,6943,612
D0,6790,8540,5140,4190,8170,7990,5450,631,4053,6423,6273,404
E1,2241,4671,2680,5410,5690,5180,3470,2271,1020,6520,50,382
F0,7880,7710,7010,3760,3440,150,1450,1440,4830,2720,2690,197
G1,0420,7850,6840,3910,6290,6570,5590,3280,8741,0371,8991,424
H0,6480,4270,3650,2240,3370,3160,2510,180,5850,6981,9411,096

25/01/2022

 1 blanc2 gm13 gd1b4 gq1b5 blanc6 gm17 gd1b8 gq1b9 blanc10 gm111 gd1b12 gq1b
A43001434300143430014343001435372189537218953721895372189120247120247120247120247
B43001434300143430014343001435372189537218953721895372189120247120247120247120247
C3393113393113393113393114557353455735345573534557353C+IgGC+IgGC+IgGC+IgG
D3393113393113393113393114557353455735345573534557353C+IgGC+IgGC+IgGC+IgG
E43001434300143430014343001435372189537218953721895372189120247120247120247120247
F43001434300143430014343001435372189537218953721895372189120247120247120247120247
G3393113393113393113393114557353455735345573534557353C+IgMC+IgMC+IgMC+IgM
H3393113393113393113393114557353455735345573534557353C+IgMC+IgMC+IgMC+IgM
 123456789101112
A1,1591,2010,2260,8061,0281,0090,7970,6181,6791,5261,4430,784
B0,6720,7720,5730,4730,240,2350,1620,1350,4380,3340,3280,424
C0,7171,1760,9660,5711,0451,0071,1340,7142,15543,9863,887
D0,1520,1630,1370,1160,2240,2010,2860,2261,4442,547
E1,390,9370,7630,611,5810,8620,7720,591,0711,0710,8490,514
F0,6960,3610,350,2560,2270,1320,1350,1130,1660,1650,1390,107
G1,1390,9310,6250,4520,8210,4330,3540,311,7522,152,9342,667
H0,1090,1140,0970,0920,1240,1120,1020,0931,0471,3592,8191,974

26/01/2022

 1 blanc2 gm13 gd1b4 gq1b5 blanc6 gm17 gd1b8 gq1b9 blanc10 gm111 gd1b12 gq1b
A484693848469384846938484693859324259324259324259324270160952701609527016095270160952
B484693848469384846938484693859324259324259324259324270160952701609527016095270160952
C5308833530883353088335308833433662433662433662433662C+ IgGC+ IgGC+ IgGC+ IgG
D5308833530883353088335308833433662433662433662433662C+ IgGC+ IgGC+ IgGC+ IgG
E484693848469384846938484693859324259324259324259324270160952701609527016095270160952
F484693848469384846938484693859324259324259324259324270160952701609527016095270160952
G5308833530883353088335308833433662433662433662433662C+ IgMC+ IgMC+ IgMC+ IgM
H5308833530883353088335308833433662433662433662433662C+ IgMC+ IgMC+ IgMC+ IgM
 123456789101112
A0,1590,1380,1430,1660,610,5220,4420,3990,5390,5020,4750,355
B0,1120,1370,1320,1110,3770,3260,3480,2680,2090,2530,2050,26
C0,2080,2990,3560,2321,1611,2080,720,521,3933,6053,5873,306
D0,170,1890,1630,1210,520,4870,3560,2890,9793,5563,5662,01
E0,1470,1570,1170,0971,3560,7450,5530,3411,0260,6890,5830,39
F0,0910,1080,1180,1290,6570,3150,2620,2010,4040,2530,2150,2
G2,5871,7721,7361,2671,861,0030,8940,5331,6331,8862,952,741
H1,6790,8380,9350,5781,110,4380,350,31,0321,0882,7681,781

27/01/2022

 1 blanc2 gm13 gd1b4 gq1b5 blanc6 gm17 gd1b8 gq1b9 blanc10 gm111 gd1b12 gq1b
A5813715813715813715813715350187535018753501875350187689845689845689845689845
B5813715813715813715813715350187535018753501875350187689845689845689845689845
C17935701793570179357017935701182810118281011828101182810C+ IgGC+ IgGC+ IgGC+ IgG
D17935701793570179357017935701182810118281011828101182810C+ IgGC+ IgGC+ IgGC+ IgG
E5813715813715813715813715350187535018753501875350187689845689845689845689845
F5813715813715813715813715350187535018753501875350187689845689845689845689845
G17935701793570179357017935701182810118281011828101182810C+ IgMC+ IgMC+ IgMC+ IgM
H17935701793570179357017935701182810118281011828101182810C+ IgMC+ IgMC+ IgMC+ IgM
 123456789101112
A1,3541,3541,3781,0120,8320,4350,4380,3610,6460,4010,340,313
B0,5770,6430,5870,7390,4240,3430,2910,4670,5240,2880,4170,185
C3,4633,2282,8542,3521,7321,7351,5221,0361,9863,7053,6683,613
D2,1941,9611,5481,0490,8540,7580,7510,8241,2313,7053,6962,12
E0,9990,5430,4190,3270,5170,2090,2090,1940,7390,3260,2520,214
F0,5860,2480,1850,1610,2740,1550,1360,1270,3690,2270,1570,158
G1,3780,8510,6770,4730,780,5590,4330,3131,4241,7113,0312,563
H0,7770,3820,3610,2870,3330,2540,2120,2190,9830,9672,7851,681

28/01/2022

 1 blanc2 gm13 gd1b4 gq1b5 blanc6 gm17 gd1b8 gq1b9 blanc10 gm111 gd1b12 gq1b
A12282011228201122820112282019607709607709607709607701189119118911911891191189119
B12282011228201122820112282019607709607709607709607701189119118911911891191189119
C1833944183394418339441833944961877961877961877961877C+ IgGC+ IgGC+ IgGC+ IgG
D1833944183394418339441833944961877961877961877961877C+ IgGC+ IgGC+ IgGC+ IgG
E12282011228201122820112282019607709607709607709607701189119118911911891191189119
F12282011228201122820112282019607709607709607709607701189119118911911891191189119
G1833944183394418339441833944961877961877961877961877C+ IgMC+ IgMC+ IgMC+ IgM
H1833944183394418339441833944961877961877961877961877C+ IgMC+ IgMC+ IgMC+ IgM
 123456789101112
A2,8571,5052,6532,3520,8590,9421,1730,8211,0861,150,8410,937
B1,4091,1331,3460,9660,4980,4460,6480,6690,5480,8290,5280,428
C1,4831,531,8491,2131,9211,7282,5891,7021,8273,6393,6043,432
D0,7170,8680,830,7991,2380,8651,1681,2071,3443,5993,4932,9
E2,4812,662,9932,7851,4140,5960,9230,6791,9590,7121,3130,802
F1,8451,4441,7651,4380,5630,2830,4560,2871,1950,4290,6070,338
G1,3061,3860,8960,6661,2591,1051,1410,8311,9841,7642,9392,26
H0,6890,6120,3740,2780,6050,4770,4240,3251,2551,1482,7311,772

01/02/2022

 1 blanc2 gm13 gd1b4 gq1b5 blanc6 gm17 gd1b8 gq1b9 blanc10 gm111 gd1b12 gq1b
A143926614392661439266143926614422041442204144220414422041751712175171217517121751712
B143926614392661439266143926614422041442204144220414422041751712175171217517121751712
C2069642069642069642069641677337167733716773371677337C+ IgGC+ IgGC+ IgGC+ IgG
D2069642069642069642069641677337167733716773371677337C+ IgGC+ IgGC+ IgGC+ IgG
E143926614392661439266143926614422041442204144220414422041751712175171217517121751712
F143926614392661439266143926614422041442204144220414422041751712175171217517121751712
G2069642069642069642069641677337167733716773371677337C+ IgGC+ IgGC+ IgGC+ IgG
H2069642069642069642069641677337167733716773371677337C+ IgGC+ IgGC+ IgGC+ IgG
 123456789101112
A0,4420,3020,3470,2620,5610,530,6120,4690,7540,6840,8260,552
B0,2480,2620,2850,270,3860,4820,3230,3570,3560,4730,5990,613
C0,7980,7040,7320,6960,5870,7740,7230,5631,6533,4863,6923,636
D0,4650,6440,4880,4320,510,5490,3670,3791,4873,513,663,251
E0,4340,3840,3320,4231,1491,0010,8720,7680,7910,5760,4650,583
F0,3360,3590,3670,2120,6040,660,4130,4540,4420,6360,6010,449
G1,0750,7290,7710,5040,3920,8520,5040,5351,5561,5572,8242,514
H0,7030,4380,3450,3880,3860,4320,2450,4581,2321,0182,2912,45
 1 blanc2 gm13 gd1b4 gq1b5 blanc6 gm17 gd1b8 gq1b9 blanc10 gm111 gd1b12 gq1b
A15417741541774154177415417749112699112699112699112691614932161493216149321614932
B15417741541774154177415417749112699112699112699112691614932161493216149321614932
C60941660941660941660941682386823868238682386C+ IgGC+ IgGC+ IgGC+ IgG
D60941660941660941660941682386823868238682386C+ IgGC+ IgGC+ IgGC+ IgG
E15417741541774154177415417749112699112699112699112691614932161493216149321614932
F15417741541774154177415417749112699112699112699112691614932161493216149321614932
G60941660941660941660941682386823868238682386C+ IgMC+ IgMC+ IgMC+ IgM
H60941660941660941660941682386823868238682386C+ IgMC+ IgMC+ IgMC+ IgM
 123456789101112
A0,1940,1190,160,1690,3860,2040,3580,3080,2130,1150,2430,374
B0,080,0940,090,10,2110,1140,1670,1620,1310,0860,1520,255
C0,2520,2320,2170,1810,1590,1570,1640,1911,1473,3213,6263,582
D0,1440,110,1050,1070,1090,1010,1220,1190,9923,0443,5653,363
E0,4450,2390,2150,1950,4740,3140,310,2010,1520,1120,1220,157
F0,1180,1110,1110,0970,160,1330,1310,1080,0820,10,0850,097
G0,3020,2220,2530,1820,3110,270,2960,1751,2881,0272,5512,176
H0,1230,0970,1060,1040,1160,1020,1210,1370,660,5082,4511,82

02/02/2022

 1 blanc2 gm13 gd1b4 gq1b5 blanc6 gm17 gd1b8 gq1b9 blanc10 gm111 gd1b12 gq1b
A15987021598702159870215987021708059170805917080591708059634503634503634503634503
B15987021598702159870215987021708059170805917080591708059634503634503634503634503
C1233356123335612333561233356217975217975217975217975C+ IgGC+ IgGC+ IgGC+ IgG
D1233356123335612333561233356217975217975217975217975C+ IgGC+ IgGC+ IgGC+ IgG
E15987021598702159870215987021708059170805917080591708059634503634503634503634503
F15987021598702159870215987021708059170805917080591708059634503634503634503634503
G1233356123335612333561233356217975217975217975217975C+ IgMC+ IgMC+ IgMC+ IgM
H1233356123335612333561233356217975217975217975217975C+ IgMC+ IgMC+ IgMC+ IgM
 123456789101112
A0,0920,0940,1090,0930,2580,2430,2760,3130,7570,7220,810,532
B0,0820,1030,1010,1060,1380,1120,1810,1150,3680,3110,4270,525
C0,3350,2910,3470,3110,5140,4640,4480,3111,1363,523,633,558
D0,1680,1620,1970,1390,2710,2130,2140,1770,6713,4723,6022,947
E0,1110,1020,0960,0880,3750,3050,3560,2670,80,7340,6750,48
F0,0670,0740,0780,0710,1770,1430,1540,1310,3480,2970,2890,198
G0,8480,630,5310,420,4780,320,2590,1930,5640,7882,3541,712
H0,3230,2270,2460,2330,2250,1120,1410,130,4050,5032,0691,043
 1 blanc2 gm13 gd1b4 gq1b5 blanc6 gm17 gd1b8 gq1b9 blanc10 gm111 gd1b12 gq1b
A41527641527641527641527618637321863732186373218637321332690133269013326901332690
B41527641527641527641527618637321863732186373218637321332690133269013326901332690
C3491913491913491913491911127097112709711270971127097C+ IgGC+ IgGC+ IgGC+ IgG
D3491913491913491913491911127097112709711270971127097C+ IgGC+ IgGC+ IgGC+ IgG
E41527641527641527641527618637321863732186373218637321332690133269013326901332690
F41527641527641527641527618637321863732186373218637321332690133269013326901332690
G3491913491913491913491911127097112709711270971127097C+ IgMC+ IgMC+ IgMC+ IgM
H3491913491913491913491911127097112709711270971127097C+ IgMC+ IgMC+ IgMC+ IgM
 123456789101112
A0,4860,4540,5470,2970,1670,1680,2280,2290,3470,4090,4330,305
B0,2640,2320,3110,2050,1110,1070,1420,1080,1650,1720,190,131
C0,920,6430,7250,4410,1980,2190,3040,1830,8413,4133,5883,421
D0,2930,3020,2960,210,1270,140,1840,1660,5913,5093,5512,082
E0,8580,7440,7540,5070,3890,3160,2050,171,0121,1751,0070,772
F0,3750,2410,2970,2180,1620,1120,1120,0870,350,3520,340,282
G0,4210,4970,4910,2710,2930,3130,2120,1690,6560,9132,391,341
H0,1790,2160,2060,1810,1290,1190,110,10,4140,432,0980,817

03/02/2022

 1 blanc2 gm13 gd1b4 gq1b5 blanc6 gm17 gd1b8 gq1b9 blanc10 gm111 gd1b12 gq1b
A13793401379340137934013793409254919254919254919254911006256100625610062561006256
B13793401379340137934013793409254919254919254919254911006256100625610062561006256
C1287013128701312870131287013514508514508514508514508C+ IgGC+ IgGC+ IgGC+ IgG
D1287013128701312870131287013514508514508514508514508C+ IgGC+ IgGC+ IgGC+ IgG
E13793401379340137934013793409254919254919254919254911006256100625610062561006256
F13793401379340137934013793409254919254919254919254911006256100625610062561006256
G1287013128701312870131287013514508514508514508514508C+ IgMC+ IgMC+ IgMC+ IgM
H1287013128701312870131287013514508514508514508514508C+ IgMC+ IgMC+ IgMC+ IgM
 123456789101112
A0,3580,3310,2880,2850,1680,1110,1540,1210,3160,220,330,369
B0,1520,1750,150,1240,1080,1250,1120,0970,1440,1640,1990,192
C0,170,2530,2130,1460,1760,2710,1980,231,4223,5123,5883,497
D0,1270,110,1120,1140,1180,1350,1320,1231,083,5353,6142,918
E0,3930,2740,2620,2290,1960,160,1760,1320,5310,2560,2250,205
F0,1540,1250,1260,1140,1060,1060,1010,0860,1610,1180,1160,097
G0,1110,1120,1150,1090,2260,1730,1640,1550,8261,2762,5381,906
H0,0750,1010,0860,0850,1120,1030,1080,090,5240,7362,2791,184
 1 blanc2 gm13 gd1b4 gq1b5 blanc6 gm17 gd1b8 gq1b9 blanc10 gm111 gd1b12 gq1b
A138484013848401384840138484015768951576895157689515768951002631100263110026311002631
B138484013848401384840138484015768951576895157689515768951002631100263110026311002631
C5805185805185805185805181638251163825116382511638251C+ IgGC+ IgGC+ IgGC+ IgG
D5805185805185805185805181638251163825116382511638251C+ IgGC+ IgGC+ IgGC+ IgG
E138484013848401384840138484015768951576895157689515768951002631100263110026311002631
F138484013848401384840138484015768951576895157689515768951002631100263110026311002631
G5805185805185805185805181638251163825116382511638251C+ IgMC+ IgMC+ IgMC+ IgM
H5805185805185805185805181638251163825116382511638251C+ IgMC+ IgMC+ IgMC+ IgM
 123456789101112
A0,2350,1620,1950,1560,7530,7790,6540,5870,2340,2250,2590,195
B0,1370,1510,1070,0890,3420,3310,3880,3630,1590,1290,1840,17
C0,5220,6180,4060,3490,4650,4470,4870,411,0443,4623,5173,442
D0,2320,2550,1860,1720,1970,240,2890,1920,813,5233,5582,601
E0,1940,1410,1460,1121,1910,5270,4230,3530,3430,3480,3380,253
F0,0950,0990,10,0870,4970,2630,2290,2040,1750,160,1760,146
G0,7190,450,4250,3341,1940,7010,5020,4180,911,0382,4411,916
H0,2870,1970,1920,1750,5480,3110,2640,2430,5320,4692,1531,192
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